| 水分和铅交互胁迫对国槐幼苗生理生化特性的影响 |
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| 引用本文： | 张青,王进鑫,邹朋,初江涛,周芙蓉.水分和铅交互胁迫对国槐幼苗生理生化特性的影响[J].农业环境科学学报,2012,31(3):476-483. |
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| 作者姓名： | 张青 王进鑫 邹朋 初江涛 周芙蓉 |
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| 作者单位： | 西北农林科技大学资源环境学院,农业部西北植物营养与农业环境重点实验室,陕西杨凌712100 |
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| 摘 要： | 为了探讨在水分和铅交互胁迫条件下,国槐(Sophora japonicaL.)幼苗抗氧化酶系统活性及渗透调节物质含量的变化,采用盆栽实验,研究不同水分处理(土壤相对含水率为40%、60%、80%、100%)及5个铅胁迫水平(0、300、500、1000、2000mg.kg-1)交互作用对抗氧化酶活性、MDA含量、可溶性糖含量和脯氨酸含量的影响。结果表明:在水分和铅的交互胁迫下,国槐幼苗叶片POD、SOD酶活性表现先升后降的趋势,与单一的水分胁迫、铅胁迫相比,交互作用对SOD、POD活性的影响规律性不明显,在一定程度上表现出协同或拮抗效应;随着水分胁迫的加剧和铅胁迫的增大,国槐幼苗叶片的MDA含量变化呈现升高的趋势。在铅含量<2000mg.kg-1条件下,交互胁迫的国槐幼苗叶片MDA含量低于单一水分胁迫处理,表明国槐在交互胁迫下受到的膜伤害比单一水分胁迫下受到的膜伤害小;国槐幼苗叶片可溶性糖含量对水分胁迫较不敏感,且随着水分胁迫的加剧和铅胁迫的增加,叶片可溶性糖含量的变化比较复杂,但总体上呈先升后降的趋势;在铅含量500mg.kg-1和40%水分胁迫处理下,可溶性糖含量显著高于对照(P<0.01),MDA含量与对照无明显差异;随着水分胁迫的加剧和铅胁迫的增大,脯氨酸含量逐渐升高;在40%水分胁迫下,重金属铅和水分胁迫对叶片中脯氨酸的积累有协同促进作用。适当的交互胁迫(铅含量300、500mg.kg-1和40%水分处理)有利于提高国槐的抗逆性。
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| 关 键 词： | 干旱 铅 国槐 交互胁迫 生理生化特性 |
Effects of Interactive Stress of Dought and Lead on Physiological and Biochemical Characteristics of Sophora japonica L.Seedlings |
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| ZHANG Qing
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WANG Jin-xin
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ZOU Peng
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CHU Jiang-tao
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ZHOU Fu-rong.Effects of Interactive Stress of Dought and Lead on Physiological and Biochemical Characteristics of Sophora japonica L.Seedlings[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2012,31(3):476-483. |
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| Authors: | ZHANG Qing WANG Jin-xin ZOU Peng CHU Jiang-tao ZHOU Fu-rong |
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| Institution: | (College of Resources and Environment,Northwest A & F University,Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China,Ministry of Agriculture,Yangling 712100,China) |
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| Abstract: | The objective of this experiment is to study changes in the antioxidant enzyme activity and the osmotic solute concentration in Sophora japonica L.seedlings grown under drought and heavy metal(Pb) stress.The pot study used four soil water contents(40%,60%,80%,100% of soil water holding capacity) and five Pb concentrations(0,300,500,1 000,2 000 mg·kg-1).The results showed that peroxidase(POD) and superoxide dismutase(SOD) activity initially increased and then decreased under the combined effects of drought and Pb stress.The combination of drought and Pb stress had less effect than only drought or Pb stress on POD and SOD activity in S.japonica seedlings.The combination of drought and Pb stress had either synergistic or antagonistic effects depending on the conditions.Malondialdehyde(MDA) concentrations gradually increased under the combination of drought and Pb stress,however the MDA concentrations in the drought plus Pb stress(<2 000 mg·kg-1) treatments were still less than those of S.japonica seedlings grown under drought stress alone.This indicated that the interactive effects of drought and Pb caused more membrane damage to S.japonica seedlings than drought stress alone.Drought stress had little effect on soluble sugar concentrations in S.japonica seedlings;however interaction between drought and Pb stress caused soluble sugar contents to increase and then decrease.The soluble sugar concentration of S.japonica seedlings grown under 500 mg·kg-1 Pb stress and 40% of soil water holding capacity was greater than that of S.japonica seedlings in the control treatment(P<0.01).In contrast,the MDA concentration of S.japonica seedlings grown under 500 mg·kg-1 Pb stress and 40% of soil water holding capacity was not significantly different from that of S.japonica seedlings in the control treatment(P>0.05).Proline concentrations gradually increased over time in the 500 mg·kg-1 Pb stress plus 40% of soil water holding capacity treatment,indicating that the combination of drought and Pb stress had synergistic effects.In summary,S.japonica was resistant to the combined effects of drought(40% of soil water holding capacity) and Pb(300,500 mg·kg-1) stress. |
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| Keywords: | drought lead Sophora japonica interaction stress physiological-biochemical characteristic |
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