| 植物化的猪α-乳清蛋白基因人工合成 |
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| 引用本文： | 芦春斌.植物化的猪α-乳清蛋白基因人工合成[J].种子,2004,23(12):3-5. |
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| 作者姓名： | 芦春斌 |
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| 作者单位： | 暨南大学生殖免疫中心,广东广州,510632 |
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| 基金项目： | 美国 United Soybean Board Domestic Program Project |
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| 摘 要： | 根据猪α-乳清蛋白基因cDNA序列设计了多对特异性引物,采用循环PCR方法人工合成了植物化的猪α-乳清蛋白基因编码区(398 bp)并对该PCR产物进行测序.DNA序列测定结果表明,采用循环PCR法扩增的产物是预期的猪α-乳清蛋白基因编码区,该植物化的猪α-乳清蛋白基因编码区可以用于植物转化,为猪α-乳清蛋白基因转基因植物研究和在转基因植物中进行动物基因表达研究奠定了基础.
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| 关 键 词： | 猪α-乳清蛋白基因 循环PCR 转基因植物 |
| 修稿时间： | 2004年8月10日 |
Synthesis of Plantified Porcine Alpha-Lactalbumin Gene |
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| Lu Chunbin.Synthesis of Plantified Porcine Alpha-Lactalbumin Gene[J].Seed,2004,23(12):3-5. |
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| Authors: | Lu Chunbin |
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| Abstract: | Several pairs of PCR primers were designed and synthesized based on the coding region of porcine alpha Lactalbumin gene. The plantified 398 bp fragment coding region of porcine alpha Lactalbumin gene was amplified with recursive PCR technique. The resulting PCR products were DNA sequenced. The plantified fragment encoding region of porcine alpha-Lactalbumin gene was successfully amplified and verified by DNA sequencing. This plantified porcine alpha-Lactalbumin gene can be cloned into binary vector to produce transgenic plants. |
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| Keywords: | Porcine alpha-Lactalbumin gene Recursive PCR technique Transgenic plants |
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