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几种玉兰亚属植物的RAPD亲缘关系分析
引用本文:王亚玲,王勇,张寿洲,崔铁成.几种玉兰亚属植物的RAPD亲缘关系分析[J].园艺学报,2003,30(3):299-302.
作者姓名:王亚玲  王勇  张寿洲  崔铁成
作者单位:(西安植物园,西安 710061; 仙湖植物园,深圳 518004)
基金项目:深圳市仙湖植物园科研基金资助项目
摘    要: 以玉兰亚属的20个材料(含种和品种)为研究对象,用RAPD技术对其亲缘关系进行分析。从55个随机引物中筛选出15个,对所有供试材料进行扩增,共获得274条DNA谱带,其中262条为多态性带。建立的基于RAPD结果的亲缘关系树形图显示玉兰亚属的基因背景非常复杂,类群划分较为困难,说明中国的玉兰亚属种质资源非常丰富。

关 键 词:玉兰  RAPD  亲缘关系
文章编号:0513-353X(2003)03-0299-04
收稿时间:2002-8-16
修稿时间:2002年10月4日

Phylogenetic Relationship among Several Subgenus Yulania Species Based on RAPD Markers
Wang Yaling ,Li Yong ,Zhang Shouzhou ,and Cui Tiecheng.Phylogenetic Relationship among Several Subgenus Yulania Species Based on RAPD Markers[J].Acta Horticulturae Sinica,2003,30(3):299-302.
Authors:Wang Yaling    Li Yong  Zhang Shouzhou  and Cui Tiecheng
Institution:Xi'an Botanical Garden,Xi'an 710061,China; Shenzhen Fairy Lake Botanical Garden,Shenzhen 518004,China)
Abstract:In this study,20 materials of subgenus Yulania(include species and cultivars)were analyzed using random amplified polymorphic DNA markers.15 primers were screened from 55 arbitrary 10-mer primers,and a total of 274 DNA bands were amplified,262 of which(95.6%)were polymorphism.According to the data,a tree diagram of phylogenetic relationship was constructed using UPGMA in MEGA.The results showed that the gene background of subgenus Yulania is very complex and it was difficult to be classified.So know that the resource of subgenus Yulania in China were abundant.
Keywords:Yulania  RAPD  Phylogenetic relationship
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