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| 氨氮胁迫对四鼻须鲤鱼肝脏GST酶活力的影响 | |
| 引用本文： | 刘洋,凌去非,王磊,王大鹏,佟广军.氨氮胁迫对四鼻须鲤鱼肝脏GST酶活力的影响[J].水产养殖,2011,32(7):13-15. |
| 作者姓名： | 刘洋 凌去非 王磊 王大鹏 佟广军 |
| 作者单位： | 1. 中国水产科学研究院水渔业研究中心,中国水产科学研究院内陆渔业生态环境和资源重点开放实验室,江苏无锡,214081;苏州大学水产研究所,江苏苏州,215123 2. 苏州大学水产研究所,江苏苏州,215123 3. 铜山县农业技术推广中心,江苏徐州,221116 |
| 基金项目： | 中国水科院内陆渔业生态环境和资源重点开放实验室开放基金，江苏省水产三项工程项目 |
| 摘 要： | 实验采用生态毒理学方法,以NH4Cl为实验药物,设置0、50、150和250mg/L实验浓度。通过对野生和养殖群体的四鼻须鲤鱼氨氮对肝脏谷胱甘肽巯基转移酶（GST）活力的影响,比较两个群体抗氨氮能力。结果表明：在相同的氨氮浓度和胁迫时间下,四鼻须鲤鱼养殖群体GST酶活力的变化趋势与野生群体大致相同。除250mg/L浓度组在胁迫5d和10d时,野生群体酶活性略低于养殖群体外,在其余时间点的野生群体酶活性均高于养殖群体,而且,在高浓度氨氮胁迫下,野生群体肝脏的GST酶活性达到峰值的时间比养殖群体更短,显示出较强的抗氨氮能力。 |
| 关 键 词： | 氨氮胁迫 四鼻须鲤鱼 野生群体 养殖群体 谷胱甘肽巯基转移酶 |
Effects of ammonia-N stress on GST activities of livers of four-naris carp |
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| Liu Yang,Ling Qufei,Wang Lei,Wang Dapeng,Tong Guangjun.Effects of ammonia-N stress on GST activities of livers of four-naris carp[J].Journal of Aquaculture,2011,32(7):13-15. | |
| Authors: | Liu Yang Ling Qufei Wang Lei Wang Dapeng Tong Guangjun |
| Institution: | 1.Key Laboratory of Ecological Environment and Resources of Inland Fisheries,Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;2.Fisheries Research Institute of Soochow University,Suzhou 215123,China;3.Agriculture Technique Extend Center of Tongshan County,Xuzhou 221116,China) |
| Abstract: | An ecotoxicological feasibility study was conducted to investigate the effects of ammonia-N（NH4Cl）（0 mg/L 、50 mg/L、150 mg/L、250 mg/L） stress on the activities of GST in livers of wild four-naris carp population and farmed four-naris carp population to ammonia-N exposure in fresh water for 15 days.The results showed that：under the same ammonia concentrations and the same stress time,the change trends of activity of GST enzyme of the wild four-naris carp population was similar to the farm four-naris carp population.The activity of GST enzymes of the wild population was higher than the farm population markedly,except for 250 mg/L groups in the 5 d and 10 d.Under the high concentration stress of ammonia,the GST activity in the livers of the wild population reached the top level faster than the farm population,it suggested that the wild four-naris carp population has a stronger resistance to ammonia stress. |
| Keywords: | Four-naris carp ammonia-N stress wild population farm population Glutathione S-transferase（GST） |
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