| 杂交鳢及其亲本同工酶的比较 |
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| 引用本文： | 刘凯,谢楠,姚桂桂,刘新轶,王宇希.杂交鳢及其亲本同工酶的比较[J].水产养殖,2011,32(10):14-17. |
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| 作者姓名： | 刘凯 谢楠 姚桂桂 刘新轶 王宇希 |
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| 作者单位： | 杭州市农业科学研究院水产研究所,浙江 杭州,310024 |
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| 基金项目： | 杭州市科技重大创新项目（20083212A04） |
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| 摘 要： | 采用聚丙烯酰胺凝胶垂直板电泳，对浙江乌鳢、广东斑鳢、浙江乌鳢（♂）×广东斑鳢（♀）杂交种3个群体，肝、肌肉、脑、脾、鳃、肾、心7种组织的乳酸脱氢酶（LDH）和苹果酸脱氢酶（MDH）2种同工酶进行了分析。结果表明：2种同工酶在各自群体内具有明显的组织特异性，在浙江乌鳢、广东斑鳢间表现出一定的差异性，其杂交后代与二者有一定的相似性，但同工酶酶谱中也出现了“互补带”和“杂交带”，符合杂交育种的一般规律。
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| 关 键 词： | 杂交鳢 杂交优势 同工酶 乳酸脱氢酶 苹果酸脱氢酶 |
Comparative study on isozyme of Ophiocephalus argus Cantor(♂), Ophiocephalus maculatus(♀)and their hybrids |
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| Liu Kai,Xie Nan,Yao Guigui,Liu Xinyi,Wang Yuxi.Comparative study on isozyme of Ophiocephalus argus Cantor(♂), Ophiocephalus maculatus(♀)and their hybrids[J].Journal of Aquaculture,2011,32(10):14-17. |
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| Authors: | Liu Kai Xie Nan Yao Guigui Liu Xinyi Wang Yuxi |
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| Institution: | , Liu Xinyi, Wang Yuxi (Fisheries Research Institut, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China) |
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| Abstract: | By means of the polyacrylamidc gradient gel electrophoresis, two isozymes （ MDH and LDH ） from seven tissues （liver, muscle,brain, spleen, gills, kidney and heart）of Ophiocephalus argus Cantor （ ♂ ） , Ophiocephalus maculates （ ♀ ） and their hybrids were studied. The results showed that two isozymes presented obvious tissue. The expression of isozymes showed there was a certain extent discrepancy between Ophiocephalus argus Cantor （ ♂ ） and Ophiocephalus maculates （ ♀ ）,and their hybrids showed similarity with both of them,but ＂hybrid band＂ and ＂complementary band＂ alse were found. The conclusion was in accord with general discipline of cross breeding. |
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| Keywords: | snakehead hybrid heterosis isozyme lactate dehydrogenase malic dehydrogenase |
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