| 乳酸杆菌的分离、鉴定及体外拮抗试验 |
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| 引用本文： | 芦珂.乳酸杆菌的分离、鉴定及体外拮抗试验[J].四川畜牧兽医,2011,38(11):24-26. |
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| 作者姓名： | 芦珂 |
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| 作者单位： | 四川省雅安职业技术学院药学检验系,四川雅安,625000 |
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| 摘 要： | 本研究利用不同配方的培养基选择性分离、纯化不同生长营养需求的菌种,然后对所分离菌株作形态观察、生理生化鉴定,再用牛津杯法进行乳酸杆菌全菌液与上清液对大肠杆菌拮抗作用的对比试验。结果分别从鸡粪、牛粪、猪粪中分离、纯化出发酵乳杆菌、发状乳杆菌、木糖乳杆菌;乳酸杆菌培养上清液的拮抗作用大于全菌液;鸡源发酵乳杆菌对猪源大肠杆菌有较好的生物拮抗作用;猪源木糖乳杆菌及牛源发状乳杆菌对猪源大肠杆菌无生物拮抗作用。
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| 关 键 词： | 乳酸杆菌 生物拮抗作用 牛津杯 |
Isolation and identification of lactobacillus and the biological antagonistic action in vitro |
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| LU Ke.Isolation and identification of lactobacillus and the biological antagonistic action in vitro[J].Sichuan Animal & Veterinary Sciences,2011,38(11):24-26. |
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| Authors: | LU Ke |
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| Institution: | LU Ke(Pharmaceutical and Inspection Department of Ya＇an Vocational and Technological College,Sichuan Ya＇an 625000,China) |
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| Abstract: | In this paper,we used culture mediums of different formula to isolate and purify the microbial strains with different growth nutrition demand,and classified these microbial strains that we had isolated,by the means of the shape observation,the physiological biochemistry reaction.Then,with the use of oxford-cup,we made a contrast experiment between lactobacillus bacterial fluid and superior-clear liquid which had the antagonistic action to E.coli.The result showed that we had isolated and purified lactobacillus fermentum,lactobacillus trichoctes and lactobacillus xylosus from chicken manure,cow dung,pig excrement;the antagonistic action to E.coli of the lactobacillus superior-clear liquid was stronger than that of the bacterial fluid;the chicken source lactobacillus fermentum had evident biological antagonistic action to the pig source E.coli;the pig source lactobacillus xylosus and the cow source lactobacillus trichoctes had no biological antagonistic action to the pig source E.coli. |
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| Keywords: | Lactobacillus Biological antagonistic action Oxford-cup |
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