| 响应面法优选红皮云杉针叶中莽草酸的匀浆提取工艺 |
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| 引用本文： | 杨磊,黄金明,刘婷婷,刘玉,马春慧,刘芳,王富济,祖元刚.响应面法优选红皮云杉针叶中莽草酸的匀浆提取工艺[J].森林工程,2010,26(6):9-13,25. |
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| 作者姓名： | 杨磊 黄金明 刘婷婷 刘玉 马春慧 刘芳 王富济 祖元刚 |
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| 作者单位： | 东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 |
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| 基金项目： | 国家"十一五"科技支撑计划项目 |
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| 摘 要： | 利用响应面分析法（Response Surface Methodology）对红皮云杉中提取莽草酸的工艺进行优化。在单因素实验基础上选取实验因素与水平,根据中心组合（Box-Benhnken）实验设计原理采用3因素3水平的响应面分析法,根据回归分析确定各工艺条件的影响因子,以莽草酸的得率和纯度为响应值作响应面。在分析各因素的显著性和交互作用后,得出红皮云杉中莽草酸匀浆提取的最佳工艺条件为：提取温度为40℃,提取时间为3 min,提取料液比为1∶10.3。在最佳条件下,莽草酸的得率可达0.84%,莽草酸的实际纯度可达6.15%。
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| 关 键 词： | 响应面分析法 红皮云杉 莽草酸 |
Extraction Technology of Shikimic Acid Homogenate from Picea koraiensis Optimized by Response Surface Methodology |
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| Yang Lei,Huang Jinming,Liu Tingting,Liu Yu,Ma Chunhui,Liu Fang,Wang Fuji,Zu Yuangang.Extraction Technology of Shikimic Acid Homogenate from Picea koraiensis Optimized by Response Surface Methodology[J].Forest Engineering,2010,26(6):9-13,25. |
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| Authors: | Yang Lei Huang Jinming Liu Tingting Liu Yu Ma Chunhui Liu Fang Wang Fuji Zu Yuangang |
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| Institution: | (Key Laboratory of Ministry of Education in Forest Plant Ecology,Northeast Forestry University,Harbin 150040) |
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| Abstract: | The extraction technology of shikimic acid from Picea koraiensis was optimized by response surface methodology.Experimental factors and levels were firstly selected based on single factor test.According to the design principle of Box-Benhnken,the response surface method with three factors was adopted,and the influence factors of each technology were determined through regression analysis,then the response surface was graphed with the extraction rate and purity of shikimic acid as the response value.After analyzing the significance and the interaction of each factor,the optimum conditions of shikimic acid homogenate extraction were concluded as follows,40℃ for the extraction temperature,3 min for the extraction time and 1∶10.3 for the solid-liquid ratio.In the optimum condition,the extraction rate could reach to 0.84%,with the actual purity of 6.15%. |
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| Keywords: | response surface methodology Picea koraiensis shikimic acid |
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