| 黑果小檗中总黄酮的超声提取及其含量测定 |
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| 引用本文： | 刘河疆,钱宗耀,王建梅,周晓龙.黑果小檗中总黄酮的超声提取及其含量测定[J].安徽农业科学,2012,40(14):8210-8211. |
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| 作者姓名： | 刘河疆 钱宗耀 王建梅 周晓龙 |
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| 作者单位： | 新疆农业科学院中心实验室,新疆乌鲁木齐,830091 |
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| 摘 要： | 目的]建立一种测定黑果小檗中总黄酮含量的方法。方法]用超声波提取黑果小檗果实中的总黄酮,然后以芦丁为标准品,采用分光光度法测定总黄酮含量。结果]芦丁浓度与吸光度的线性关系方程为A=0.010 74 C+0.009 214(r=0.999 2),表明芦丁在7.5～60.0μg/ml范围内与吸光度值线性关系良好,平均回收率为93.6%;测得黑果小檗果肉、籽粒中总黄酮的含量分别为0.78%和1.89%。结论]该方法快速、简便、可靠,可用于黑果小檗中总黄酮含量的测定。
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| 关 键 词： | 黑果小檗 总黄酮 芦丁 分光光度法 超声提取 |
Extraction of Total Flavonoids in Breberis heteropoda Shrenk by Ultrasonic and Determination of Its Content |
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| Institution: | LIU He-jiang et al(Center Laboratory of Xinjiang Academy of Agricultural Science,Urmqi,Xinjiang 830091) |
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| Abstract: | Objective] The aim was to establish a method to determine total flavonoids content in Breberis heteropoda Shrenk.Method] Ultrasonic technology was used to extract the total flavonoid of the fruits of B.heteropoda Shrenk,and then with rutin as the standard substance,total flavonoids were determined by spectrophotometry.Result] The results showed that the linear relationship equation of rutin concentration and the absorbance was A=0.010 74 C+0.009 214(r=0.999 2),which indicated that there was a good linear relationship between the absorbance and the concentration of rutin within the range of 7.5~60.0 μg/ml.The average recovery rate was 93.6%,and the contents of total flavonoids in pulp and seed were 0.78% and 1.89% respectively.Conclusion] The method was found to be fast,simple,reliable and good for the determination of total flavonoids in B.heteropoda Shrenk. |
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| Keywords: | Breberis heteropoda Shrenk Total flavonoids Rutin Spectrophotometry Ultrasonic extraction |
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