| 响应面法优化霍山石斛多糖提取工艺 |
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| 引用本文： | 张炜玲,王新生,戴亚峰,李耀亭.响应面法优化霍山石斛多糖提取工艺[J].安徽农业科学,2012(12):7061-7064. |
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| 作者姓名： | 张炜玲 王新生 戴亚峰 李耀亭 |
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| 作者单位： | 1. 安徽圣农生物科技股份有限公司,安徽六安,237012
2. 安徽圣农生物科技股份有限公司,安徽六安237012;安徽省植物细胞工程技术研究中心,安徽六安237012 |
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| 基金项目： | 国家科学技术部农业科技成果转化重点项目 |
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| 摘 要： | 目的]优化霍山石斛多糖的提取工艺。方法]通过单因素试验,研究了浸提时间、料液比和乙醇浓度对霍山石斛多糖提取的影响;通过3因素3水平的响应面分析法,分析了各工艺参数与响应值之间的关系,并由此预测并验证最佳的工艺条件。结果]霍山石斛多糖提取的最佳工艺条件为:料液比为1∶340(g/ml),提取时间为3.25 h,沉淀多糖的乙醇浓度为86%;该条件下霍山石斛多糖的理论提取率为39.10%,实测值为38.78%,与预测值接近。结论]采用响应面法优化得到的提取条件准确可靠,并且提取率较高,为制剂生产提供了参考。
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| 关 键 词： | 霍山石斛 多糖 响应面分析 |
Optimization of Extraction Process for Polysaccharides from Dendrobium huoshanense by Response Surface Methodology |
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| Institution: | ZHANG Wei-ling et al(Anhui Sannor Biotechnology Co.,Ltd.,Lu’an,Anhui 237012) |
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| Abstract: | Objective] To optimize the extraction process of polysaccharides from Dendrobium huoshanense.Method] Effects of extraction time,solid-liquid ratio and ethanol concentration on the extraction rate of polysaccharides from D.huoshanense were studied by single factor test.Relationship between the process parameters and response value was studied by response surface methodology.And the optimal extraction process was forecasted.Result] The optimal extraction process of polysaccharides from D.huoshanense was 1∶ 340(g/ml) solid-liquid ratio,3.25 h extraction time and 86% ethanol concentration.Under this condition,the theoretical extraction rate of polysaccharides from D.huoshanense reached 39.10%.And the measured value was 38.78%,which was close to the predictive value.Conclusion] Extraction process optimized by response surface methodology was accurate and reliable with high extraction rate,which provided reference for the production of preparations. |
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| Keywords: | Dendrobium huoshanense Polysaccharide Response surface methodology |
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