| 葱蝇ADH基因的克隆及序列分析 |
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| 引用本文： | 陈春露,陈斌,司风玲,何正波.葱蝇ADH基因的克隆及序列分析[J].安徽农业科学,2012,40(14):8027-8030. |
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| 作者姓名： | 陈春露 陈斌 司风玲 何正波 |
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| 作者单位： | 重庆师范大学昆虫与分子生物学研究所,重庆高校生物活性物质工程研究中心,重庆市动物生物学重点实验室,四川重庆401331 |
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| 基金项目： | 国家自然科学基金，重庆市教委科学技术研究项目，重庆师范大学校级基金重点项目 |
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| 摘 要： | 目的]对葱蝇(Delia antiqua)ADH基因进行克隆,并对其进行序列分析。方法]通过RACE的方法克隆葱蝇ADH基因的cDNA序列,同时对该序列进行同源性分析、氨基酸序列比对和系统发育分析。结果]试验获得的cDNA全长1 088 bp,其中ORF 771 bp,编码256个氨基酸,推测其相对分子质量为30.80 kDa,等电点为8.22;通过该基因推导的氨基酸序列与其他物种的ADH进行相似性比较和系统发育分析,发现葱蝇与刺舌蝇(Glossina morsitans)氨基酸序列的同源性最高。结论]该研究为ADH基因的进一步研究提供了基础。
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| 关 键 词： | 葱蝇 乙醇脱氢酶 ADH基因 序列分析 系统发育 |
cDNA Cloning and Sequence Analysis of ADH Gene in Delia antiqua |
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| Institution: | CHEN Chun-lu et al ( Chongqing Key Laboratory of Animal Biology,Chongqing Engineering Research Center of Bioactive Substances,Institute of Entomology and Molecular Biology,Chongqing Normal University,Chongqing 401331) |
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| Abstract: | Objective]The aim was to clone the ADH gene of Delia antiqua,and carry out a sequence analysis.Method]The cDNA sequence of ADH gene was cloned with the method of RACE,and then studied with homology analysis,comparison of amino acid sequence and phylogenetic analysis.Result]The full length of cDNA obtained was 1 088 bp,among which there were 771 bp of ORF,encoding a protein of 256 amino acids with a calculated molecular weight of 30.80 kKa and a theoretical isolectric point of 8.22.The deduced amino acid sequence had the highest identity with that of Glossina morsitans based on homological analysis,and a phylogenic tree was inferred with homological ADH sequences from other insects.Conclusion]The study provides a basis for the further research of ADH gene. |
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| Keywords: | Delia antiqua Alcohol dehydrogenase ADH gene Sequence analysis Phylogeny |
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