| 扁玉螺多糖的提取及含量测定 |
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| 引用本文： | 杨红丽,刘辉,俞群娣,韩凯宁,夏松养.扁玉螺多糖的提取及含量测定[J].安徽农业科学,2012(13):7706-7707,7709. |
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| 作者姓名： | 杨红丽 刘辉 俞群娣 韩凯宁 夏松养 |
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| 作者单位： | 浙江海洋学院食品与药学学院,浙江舟山,316000;浙江海洋学院普陀科学技术学阮,浙江舟山,316100 |
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| 摘 要： | 目的]对扁玉螺多糖的提取条件进行优化并测定多糖的含量。方法]采用苯酚-硫酸比色法测定多糖的含量,以多糖的得率为指标,在单因素分析的基础上进行正交分析,优化扁玉螺多糖的提取工艺条件。结果]最佳提取条件为:提取温度40℃,料液比1∶100(g/ml),提取时间60 min;在此提取条件下,扁玉螺多糖的提取率为7.17%,RSD为1.05%。结论]该方法简便可靠,为扁玉螺多糖的进一步开发利用提供了依据。
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| 关 键 词： | 扁玉螺(Neverita didyma) 多糖 提取 测定 |
Extraction and Content Determination of Polysaccharides from Neverita didyma |
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| Institution: | YANG Hong-li et al(College of Food and Pharmaceutical Science,Zhejiang Ocean University,Zhoushan,Zhejiang 316000) |
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| Abstract: | Objective] To optimize the extraction conditions and determine the content of polysaccharide from Neverita didyma.Method] The polysaccharide content was determined by phenol-sulfuric acid method,with the yield of polysaccharide as index,an orthogonal analysis was carried out based on single factor analysis to optimize the extraction conditions of polysaccharide from Neverita didyma.Result] The optimum extraction parameters were:extraction temperature of 40 ℃,extraction duration of 60 min,solid-liquid ratio of 1:100.Under the best conditionsy the extraction rate of Neverita didyma polysaccharide was 7.17% with RSD of 1.05%.Conclusion] The method is simple and reliable,thus providing basis for the further development and utilization of Neverita didyma polysaccharide. |
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| Keywords: | Neverita didyma Polysaccharide Extraction Determination |
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