| 胡萝卜幼苗根、茎、叶组织培养再生植株 |
| |
| 引用本文： | 张唯,王伟,续晨,贲爱玲,张君艳,蔡小宁.胡萝卜幼苗根、茎、叶组织培养再生植株[J].安徽农业科学,2012(12):7013-7014. |
| |
| 作者姓名： | 张唯 王伟 续晨 贲爱玲 张君艳 蔡小宁 |
| |
| 作者单位： | 南京晓庄学院,江苏南京,211171;南京晓庄学院,江苏南京,211171;南京晓庄学院,江苏南京,211171;南京晓庄学院,江苏南京,211171;南京晓庄学院,江苏南京,211171;南京晓庄学院,江苏南京,211171 |
| |
| 基金项目： | 江苏省大学生实践创新训练计划项目，南京市重点学科建设项目 |
| |
| 摘 要： | 目的]研究胡萝卜幼苗的组织培养再生技术。方法]以培养约2个月的胡萝卜幼苗为材料,分别取幼苗的根、茎、叶为外植体,研究不同培养基、不同外植体对愈伤组织诱导的影响,以及培养基对愈伤组织分化的影响。结果]根、茎、叶为外植体均能产生愈伤组织,但以茎为外植体诱导的愈伤组织质量最佳,其愈伤组织的诱导率和分化率均为最高。结论]建立了胡萝卜试管培养幼苗的愈伤组织诱导和再生植株体系,为今后的试验打下了基础。
|
| 关 键 词： | 胡萝卜 幼苗 组织培养 再生植株 |
Tissue Culture and Plant Regeneration of the Root, Stem and Leaf of Carrot Seedlings |
| |
| Institution: | ZHANG Wei et al(Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171) |
| |
| Abstract: | Objective] The aim was to study plant regeneration of carrot.Method] The root,stem and leaf of two month old seedlings were taken as explants.Effect of different medium and different explants on callus induction and callus differentiation was analyzed.Result] The three explants could induce callus.The stem as explants was the best for callus quality,its rate of callus induction and differentiation rate were the highest.Conclusion] The callus induction and regeneration system of carrot seedlings was established,which lays foundation for the future experiments. |
| |
| Keywords: | Carrot Seedling Tissue culture Plant regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
