| 苎麻SSR反应体系的优化 |
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| 引用本文： | 蒋杰,佘玮,邢虎成,揭雨成.苎麻SSR反应体系的优化[J].安徽农业科学,2012,40(17):9206-9208. |
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| 作者姓名： | 蒋杰 佘玮 邢虎成 揭雨成 |
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| 作者单位： | 湖南农业大学苎麻研究所,湖南长沙,410128;湖南农业大学苎麻研究所,湖南长沙410128;湖南省种质资源创新与资源利用重点实验室,湖南长沙410128 |
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| 摘 要： | 目的]优化苎麻SSR反应体系。方法]以苎麻湘苎2号基因组DNA为模板,对其SSR反应体系中的部分影响因素进行探讨分析,并利用选定的7个苎麻品种基因组DNA为模板,对优化的SSR反应体系进行试验验证。结果]在25μl SSR反应体系中,模板量为50 ng,Mg2+浓度为1.5 mmol/L,dNTPs浓度为0.10 mmol/L,引物浓度为0.2μmol/L,Taq DNA聚合酶用量为1 U时,反应体系为最佳。利用该反应体系,对7个苎麻品种进行的SSR反应结果显示不同品种间DNA谱带多态性丰富,同时表明优化的反应体系重复性和稳定性良好。结论]该研究结果为应用SSR技术进行苎麻种质资源的研究奠定良好的基础。
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| 关 键 词： | 苎麻 SSR 反应体系 优化 |
Optimization of the SSR Reaction System of Boehmeria nivea(L.)Gaud |
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| Institution: | JIANG Jie et al(Research Institute of Ramie,Hunan Agricultural University,Changsha,Hunan 410128) |
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| Abstract: | Objective] To optimize the reaction system of B.nivea.Method] With the genomic DNA of Xiangzhu No.2 as the template,the influencing factors of its SSR reaction system were analyzed,and then the optimized SSR reaction system was experimentally verified with the genomic DNA of seven B.nivea varieties as template.Result] The 25 μl SSR reaction system containing 50 ng DNA template,1.5 mmol/L MgCl2,0.10 mmol/L dNTPs,0.2 μmol/L primer,and 1 U Taq DNA polymerase was the best.By using the reaction system,the SSR reaction results of seven B.nivea cultivars showed abundant polymorphism between different cultivars,and the optimized reaction system had good repeatability and stability.Conclusion] The experimental results lay foundation for the study of B.nivea resources by SSR technology. |
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| Keywords: | Boehmeria nivea(L )Gaud SSR Reaction system Optimization |
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