| HPLC法测定眼保Ⅰ号胶囊中葛根素和芍药苷的含量 |
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| 引用本文： | 谭家华,罗君,贺祝英,张建玲,于佳.HPLC法测定眼保Ⅰ号胶囊中葛根素和芍药苷的含量[J].安徽农业科学,2012(12):7067-7068,7071. |
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| 作者姓名： | 谭家华 罗君 贺祝英 张建玲 于佳 |
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| 作者单位： | 贵阳中医学院第一附属医院,贵州贵阳,550001 |
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| 基金项目： | 贵州省中医药管理局中医药、民族医药科学技术课题研究项目 |
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| 摘 要： | 目的]建立眼保Ⅰ号胶囊中葛根素和芍药苷的含量测定方法。方法]色谱柱为Diamonsil C18(2)(4.6 mm×250 mm,5μm);流速为1 ml/min;柱温为25℃;葛根素流动相为无水甲醇-水(28∶72,V/V),检测波长为250 nm;芍药苷流动相为乙腈-浓度0.1%磷酸溶液(14∶86,V/V),检测波长为230 nm。结果]葛根素在0.001 18～0.007 08μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.999 9),平均回收率102.4%,RSD为1.17%(n=6);芍药苷在0.044～0.261μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.999 9),平均回收率97.8%,RSD为1.71%(n=6);眼保Ⅰ号胶囊内容物,葛根素和芍药苷的平均含量分别为0.055和0.168 mg/g。结论]该方法简单,准确,重现性好,可用于眼保Ⅰ号胶囊中葛根素和芍药苷的含量检测。
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| 关 键 词： | HPLC 眼保Ⅰ号胶囊 葛根素 芍药苷 |
Determination of Puerarin and Paeoniflorin in Yanbao I Capsule by HPLC |
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| Institution: | TAN Jia-hua et al(The first Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550001) |
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| Abstract: | Objective] To establish the method for determining puerarin and paeoniflorin in Yanbao I capsule.Method] The HPLC analysis used chromatography glass column Diamonsil C18(2)(4.6 mm×250 mm,5 μm) with the detection wavelength at 250 nm for puerarin and 230 nm for paeoniflorin and the column temperature was set at 25 ℃.Following the injection of sample solution,an elution was initiated at a flow rate of 1 ml/min with anhydrous methanol-water(V/V,28∶ 72) for Puerarin and acetonitrile-0.1% phosphoric acid(V/V,14∶ 86) as mobile phase.Result] The linear range of puerarin was from 0.001 18 to 0.007 08 μg/ml(r=0.999 9).The average recovery of sample was 102.4% with RSD of 1.17%(n=6).The linear range of paeoniflorin was from 0.044 to 0.261 μg/ml(r=0.999 9).The average recovery of sample was 97.8% with RSD of 1.71%(n=6).In Yanbao I capsule,the content of puerarin and paeoniflorin could reach 0.055 and 0.168 mg/g,respectively.Conclusion] The method is simple,accurate and repeatable,which is suitable for determining puerarin and paeoniflorin in Yanbao I capsule. |
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| Keywords: | HPLC Yanbao I capsule Puerarin Paeoniflorin |
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