| 白酒丢糟纤维素降解菌的筛选 |
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| 引用本文： | 程驰,吴华昌,刘小俊,杨欣怡,周祥,范海波.白酒丢糟纤维素降解菌的筛选[J].安徽农业科学,2012(13):7892-7893. |
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| 作者姓名： | 程驰 吴华昌 刘小俊 杨欣怡 周祥 范海波 |
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| 作者单位： | 1. 四川理工学院生物工程学院,四川自贡643000;四川大学生物资源与生态环境教育部重点实验室,四川大学生命科学学院,四川成都610064
2. 四川理工学院生物工程学院,四川自贡,643000 |
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| 基金项目： | 四川省教育厅项目，四川省自贡市科技局重点科技计划项目，四川理工学院“大学生创新基金重点项目” |
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| 摘 要： | 目的]筛选分解白酒丢糟纤维素能力强的菌株。方法]首先通过采样、培养,测算水解圈与菌落直径比值和滤纸减重率进行初筛,再通过酶活测定复筛得到酶解纤维素能力强的菌株,然后进行固态产酶发酵测定其对丢糟的实际降解和产酶能力。结果]初筛出23株产纤维素酶菌株,复筛出酶活最强的5株真菌;5株真菌对酒糟的降解能力均较强。结论]从白酒丢糟筛选出的5株真菌可作为降解丢糟纤维素的优良菌株;该研究为白酒丢糟的综合利用提供了依据。
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| 关 键 词： | 白酒丢糟 纤维素降解菌 酶活 固态发酵 |
Study on Screening of Strains Decomposing Cellulose in the Distiller' s Grains |
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| Institution: | CHENG Chi et al(College of Bioengineering,Sichuan University of Science & Engineering,Zigong,Sichuan 643000) |
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| Abstract: | Objective] To screen the strains with strong capacity to decompose the cellulose in the distiller’s grains.Method] The cellulose-decomposing strains were firstly screened out by sampling,culture,the diameter ratios of hydrolysis circle to colony and the degradation ratios of filter paper,and then re-screened through the measurement of enzymatic activities.Their practical cellulose-degrading and producing capacities were measured through solid-state fermentation.Result] 23 cellulose-producing strains were primarily screened out,and five of them were left after re-screening,all the five strains showed strong capacity to decompose the cellulose in the distiller’s grains.Conclusion] The five strains perform well in decomposing the cellulose of the distiller’s grain,and the study provides basis for the comprehensive utilization of the distiller’s grain. |
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| Keywords: | Distiller’s grains Cellulose-decomposing strains Enzyme activity Solid-state fermentation |
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