| 烟草苯丙氨酸解氨酶活力与多酚含量的关系研究 |
| |
| 引用本文： | 邵伏文,薛宝燕,郭家明,陈学平.烟草苯丙氨酸解氨酶活力与多酚含量的关系研究[J].安徽农业科学,2012(12):7009-7011. |
| |
| 作者姓名： | 邵伏文 薛宝燕 郭家明 陈学平 |
| |
| 作者单位： | 1. 安徽省烟草公司,安徽合肥,230022
2. 中国科学技术大学烟草与健康研究中心,安徽合肥,230051 |
| |
| 摘 要： | 目的]测定不同品种烟草幼苗中苯丙氨酸解氨酶(PAL)活力、比活力以及多酚含量,研究PAL活力与多酚含量的关系。方法]3个烟草品种分别为西1#、东2#和中3#。结果]不同酶提取缓冲液对测定的PAL活力值有影响,其中10%PVP的效果最好;烟草幼苗中的多酚含量高,则其PAL活力及比活力都较高,而且PAL活力与酶提取液中β-巯基乙醇的浓度呈正向相关关系。在试验材料中,多酚含量没有达到抑制PAL活力的水平。结论]该研究可为探索改进PAL活力测定方法,明确多酚含量与PAL酶活力间的关系提供参考依据。
|
| 关 键 词： | PAL 活力 比活力 多酚含量 |
Study on the Relationship between Activity of Phenylalanine Ammonia Lyase (PAL) and Polyphenol Content in Tobacco Seedling |
| |
| Institution: | SHAO Fu-wen et al(Tobacco Company of Anhui Province,Hefei,Anhui 230022) |
| |
| Abstract: | Objective] The purpose was to determine the activity of phenylalanine ammonia lyase(PAL),specific activity and polyphenol content in different varieties of tobacco seedling,and study the relationship between PAL activity and polyphenol content.Method] Three tobacco variety was west 1#,east 2# and middle 3#,respectively.Result] The enzyme extraction buffers had some effects on PAL activity value,in which 10%PVP’s effects was the best.The PAL activity of the tobacco variety seedling with higher polyphenol content was higher,and PAL activity showed a positive correlation with the concentration of β-mercaptoethanol in enzyme extraction buffer.Polyphenol content could hardly inhabit the activity of PAL.Conclusion] The study provides a reference basis for exploring modified determination method of PAL activity,and confirm the relationship between PAL activity and polyphenol content. |
| |
| Keywords: | PAL Activity Specific activity Polyphenol content |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
