Isolation and characterization of 28 polymorphic SSR loci from castor bean (<Emphasis Type="Italic">Ricinus communis</Emphasis> L.) |
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| Authors: | Kyoung-In Seo Gi-An Lee Kyung-Ho Ma Do-Yoon Hyun Yong-Jin Park Jong-Wook Jung Sok-Young Lee Jae-Gyun Gwag Chung-Kon Kim Myung-Chul Lee |
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| Institution: | (1) Plant Genetic Resources Conservation Unit, USDA-ARS, 1109 Experiment Street, Griffin, GA 30223, USA; |
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| Abstract: | Castor bean (Ricinus communis) is cultivated for seed oil throughout tropical and subtropical regions but the understanding of its genetic variability
is limited. Because applicable microsatellite markers are not sufficient, we isolated and characterized polymorphic simple
sequence repeat (SSR) loci acquired from a microsatellite-enriched genomic DNA library of castor bean. Finally, 28 SSR loci
revealed polymorphisms in a castor bean collection consisting of 72 accessions. A total of 73 alleles were detected, with
an average of 3.18 alleles per locus, and the polymorphism information content (PIC) ranged from 0.03 to 0.47 (mean = 0.26).
Values for observed (HO) and expected (HE) heterozygosity ranged from 0.00 to 0.19 (mean = 0.11) and from 0.04 to 0.54 (mean = 0.31), respectively. To understand genetic
relationships within the castor bean collection, a dendrogram was constructed based on profiles of the 28 SSR loci. These
newly developed SSRs will be useful tools for assessing genetic diversity and population structure in castor bean. |
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