| 不同消毒剂对甘肃红豆草种子消毒效果及萌发的影响 |
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| 引用本文： | 董文科,马晖玲,陈春艳.不同消毒剂对甘肃红豆草种子消毒效果及萌发的影响[J].草原与草坪,2015(4):80-83. |
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| 作者姓名： | 董文科 马晖玲 陈春艳 |
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| 作者单位： | 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃 兰州,730070 |
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| 基金项目： | 甘肃省科技厅科技支撑计划项目 |
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| 摘 要： | 利用正交设计试验研究了3个不同种子预浸泡时间(6,12和24h),3种消毒剂(10%H2O2,30%NaClO和0.1%HgCl2)和3个不同消毒时间(10,20和30min)对甘肃红豆草种子萌发率、污染率及发芽指数的影响,以获取甘肃红豆草无菌苗时种子的最佳消毒方法。结果表明,3个因素对发芽率影响差异显著;预浸泡时间对发芽指数影响差异极显著,而对污染率影响差异不显著;消毒剂种类及消毒时间对污染率和发芽指数影响差异显著。综合各项因素,室温条件下无菌水预浸泡24h、0.1%HgCl2消毒20min种子发芽率较高,污染率较低,发芽较快且出苗整齐,是获取甘肃红豆草无菌苗种子的最佳消毒方式。
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| 关 键 词： | 消毒剂 红豆草 萌发 污染 |
Effects of different disinfectants on seed sterilization and germination of Onobrychis viciaefolia cv.Gansu |
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| Abstract: | Experiments with orthogonal design were carried out to explore an optimal sterilizing method for Onobrychis viciaefolia seeds through pre-soaking (6,12,24 h)and different disinfectants (10% H 2 O2 ,30% Na-ClO,0.1% HgCl2 )and sterilizing time (10,20,30 min),The germination,contamination rate and germination index of were measured.The results showed that all factors had the significant effects on germination;the pre-soaking time had the most significant effects on germination,but it was not significant on contamination .The disinfectants and sterilizing time had significant effects on contamination and germination .Therefore,the best treatment was pre-soaking in sterilized distilled water for 24 h at room temperature,and then to treat the seed with 0.1% (w/v)aqueous mercuric chloride for 20 min . |
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| Keywords: | disinfectants Onobrychis viciaefolia germination contamination |
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