| 秤锤树离体培养和植株再生 |
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| 引用本文： | 蒋泽平,梁珍海,刘根林,吴纲.秤锤树离体培养和植株再生[J].园艺学报,2005,32(3):537-540. |
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| 作者姓名： | 蒋泽平 梁珍海 刘根林 吴纲 |
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| 作者单位： | (江苏省林业科学研究院, 南京211153) |
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| 基金项目： | 江苏省科技厅社会公益与服务专项资金资助项目(BM2001701) |
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| 摘 要： | 在经选育的5年生秤锤树优良母株上, 取其腋芽茎段和顶芽为外植体, 进行离体培养和植株再生研究。结果表明, 以改良MS +BA 1.0 mg/L + TDZ 0.05 mg/L + IBA 0.05 mg/L +维生素C 50 mg/L + 3%蔗糖最适宜芽苗增殖; 改良MS +BA 0.5 mg/L + TDZ 0.05 mg/L + IBA 0.1 mg/L +维生素C 50 mg/L +3%蔗糖最适宜芽苗壮苗生长; 最适宜生根培养基为1 /2MS + IBA 0.5 mg/L +NAA 0.3 mg/L +维生素C 100 mg/L + 2%蔗糖; 经生根的试管苗移栽于蛭石∶珍珠岩∶泥炭= 6∶3∶1的混合基质, 控制温度20～30℃, 相对湿度85% ～90% , 保湿15～20 d, 其间适当遮荫, 35 d后成活率达92%以上。
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| 关 键 词： | 秤锤树 离体培养 植物生长调节剂 维生素C |
| 文章编号： | 0513-353X(2005)03-0537-04 |
| 收稿时间： | 2004-4-16 |
| 修稿时间： | 2004-11-22 |
In Vitro Culture and Plant Regeneration of Sinojackia xylocarpa Hu |
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| Jiang Zeping,Liang Zhenhai,Liu Genlin,Wu Gang.In Vitro Culture and Plant Regeneration of Sinojackia xylocarpa Hu[J].Acta Horticulturae Sinica,2005,32(3):537-540. |
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| Authors: | Jiang Zeping Liang Zhenhai Liu Genlin Wu Gang |
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| Institution: | Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China |
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| Abstract: | In order to protect and utilize effectively the endangered species Sinojackia xylocarpa Hu, orthogonal testswere designed to get the effects of plant hormones on the multip lication, growth and rooting of the species tube-shoots. A study was conducted on in vitro culture and plant regeneration of Sinojackia xylocarpa Hu with the nodal segments or apical buds excised from the newly-grown shoots of the five-year-old elite maternal plant. Results showed that the modified MS supplemented with BA 1.0 mg/L + TDZ 0.05 mg/L +IBA 0.05 mg/L +Vitamin C 50 mg/L + 3% Sucrose was optimal for the tube-shoot multiplication while the modifiedMS with BA 0.5 mg/L + TDZ 0.05 mg/L + IBA 0.1 mg/L +Vitamin C 50 mg/L + 3% Sucrose was suitable for the vigorous growth. 1 /2MS with IBA 0.5 mg/L +NAA 0.3 mg/L +Vitamin C 100 mg/L + 2%Sucrose op timized the tube-shoot rooting induction. The plantlets with root were transplanted into the substrate containing vermiculate, pearlite and peat, 6∶3∶1 ( v/v). At 20 - 30℃, relative humidity of 85% - 90% for 15 - 20 days, the plantlets could grow well, with the survival up to 92% after 35 days’ transplanting. |
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| Keywords: | Sinojackia xylocarpa Hu In vitro culture Plant hormones Vitamin C |
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