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| 黄花倒水莲(Polygala fallax Hemsl)组培快繁技术研究 | |
| 引用本文： | 刘秀芳,林文革,苏明华,陈绍煌,吴美华.黄花倒水莲(Polygala fallax Hemsl)组培快繁技术研究[J].种子,2012,31(2):57-59,63. |
| 作者姓名： | 刘秀芳 林文革 苏明华 陈绍煌 吴美华 |
| 作者单位： | 1. 地缘(厦门)生物科技有限公司,厦门,363000 2. 福建省亚热带植物研究所,厦门,363006 3. 福建省三明林业学校,福建三明,366000 |
| 摘 要： | 以当年生黄花倒水莲幼嫩带腋芽茎段作为外植体,开展组织培养试验研究.结果表明:最佳外植体诱导培养基为1/2 MS+ BA 2.0 mg/L+ NAA 0.1 mg/L,诱导率可达95.1％;最佳增殖培养基为WPM+ BA 1.5 mg/L+ NAA0.1 mg/L,增殖系数为6.12,周期为25 d,不定芽生长状况好;最佳生根培养基为1/2 WPM+ IBA 0.1 mg/L+ ABT0.4 mg/L,生根率为95.67％;以泥炭土∶黄泥土∶珍珠岩(2∶1∶1)为移栽基质,移栽成活率可达92.6％,苗木长势好,叶色绿. |
| 关 键 词： | 黄花倒水莲 药用植物 组织培养 快繁 |
Study on Tissue Culture Techniques for Polygala fallaxHemsl |
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| LIU Xiu-fang , LIN Wen-ge , SU Ming-hua , CHEN Shao-huang , WU Mei-hua.Study on Tissue Culture Techniques for Polygala fallaxHemsl[J].Seed,2012,31(2):57-59,63. | |
| Authors: | LIU Xiu-fang LIN Wen-ge SU Ming-hua CHEN Shao-huang WU Mei-hua |
| Institution: | 1(1.Diyuan(Xiamen) Biotechnology Company Ltd.,Xiamen 361006,China; 2.Fujian Institute of Subtropical Botany,Xiamen 361006,China; 3.Fujian Sanming Forestry School,Sanming Fujian 366000,China) |
| Abstract: | Stems with axillary buds from Polygala fallax Hemsl were used as explants for tissue culture.The results showed that the suitable medium of explants inducing was 1/2 MS supplemented with BA 2.0 mg/Land NAA 0.1 mg/L,and the inducing rate was 95.1%.The WPM supplemented with BA 1.5 mg/L and NAA 0.1 mg/Lwas the optimized proliferating medium with multiplication coefficient being 6.12 and the growth cycle being 25 d,and the shoots grew well.The best rooting medium was 1/2 WPM supplemented with IBA 0.1 mg/L and ABT 0.4 mg/L,and the rooting rate was 95.67%.The plantlets were transplanted in mixture media with peat,yellow soil and perlite(2∶1∶1),and the survival rate was 92.6%. |
| Keywords: | Polygala fallax Hemsl medicinal plant tissue culture rapid propagation |
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