| 内参基因在虾夷扇贝定量PCR中表达稳定性的比较 |
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| 引用本文： | 鲍相渤,刘卫东,姜冰,苏浩,李云峰,单忠国,于赫男,赫崇波.内参基因在虾夷扇贝定量PCR中表达稳定性的比较[J].水产科学,2011,30(10):603-608. |
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| 作者姓名： | 鲍相渤 刘卫东 姜冰 苏浩 李云峰 单忠国 于赫男 赫崇波 |
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| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁大连 116023
2. 辽宁师范大学生命科学学院,辽宁大连,116029 |
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| 基金项目： | 国家海洋公益性行业科研专项，辽宁省科学技术计划项目 |
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| 摘 要： | 应用实时荧光定量技术,检测虾夷扇贝的β-actin、Glyceraldehydes-3-phosphate dehydrogen-ase、α-tubulin、Cytochrome b5、TATA box binding protein-associated factor、Elongation factor 1α共6个内参基因在饥饿胁迫下各组织、致病菌感染前后和水环境升温前后不同时间段血样中的mRNA表达情况。经geNorm程序统计分析,6个内参基因在上述处理中的表达稳定性存在差异,导致不同处理中适用的内参基因有所不同。为进一步开展虾夷扇贝基因表达的定量研究奠定了基础。
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| 关 键 词： | 内参基因 geNorm程序 基因表达 实时定量PCR 虾夷扇贝 |
Expression Stability of Reference Genes for Quantitative PCR in Japanese Scallop Patinopecten yessoensis |
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| BAO Xiang-bo,LIU Wei-dong,JIANG Bing,SU Hao,LI Yun-feng,SHAN zhong-guo,YU He-nan,HE Chong-bo.Expression Stability of Reference Genes for Quantitative PCR in Japanese Scallop Patinopecten yessoensis[J].Fisheries Science,2011,30(10):603-608. |
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| Authors: | BAO Xiang-bo LIU Wei-dong JIANG Bing SU Hao LI Yun-feng SHAN zhong-guo YU He-nan HE Chong-bo |
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| Institution: | 1(1.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Key Lab of Marine Fishery Molecular Biology,Dalian 116023,China;2.College of Life Science,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | The selection of reference genes is one of the most important steps in the analysis of gene expression.Here the authors assessed the expression variation and stability of six reference genes(actin,gapdh,tubulin,cytb,tbp and elong) in different tissues of Japanese scallop Patinopecten yessoensis under starvation,and the stability after infection and temperature rise were also evaluated with real-time PCR.Differences in expression stability were evaluated by analysis of geNorm program.The most stable reference genes differed in different experimental conditions.In conclusion,the necessity of choosing proper reference genes was proved,and the systemic comparison of expression stability among these reference genes provided a foundation with the gene expression investigation of Japanese scallop. |
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| Keywords: | reference gene geNorm gene expression real-time PCR Patinopecten yessoensis |
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