| 采用种间特异性PCR对海参产品的物种鉴定研究 |
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| 引用本文： | 文菁,胡超群,张吕平,范嗣刚.采用种间特异性PCR对海参产品的物种鉴定研究[J].水产科学,2011,30(12):768-773. |
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| 作者姓名： | 文菁 胡超群 张吕平 范嗣刚 |
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| 作者单位： | 1. 湛江师范学院生命科学与技术学院,广东湛江524048;中国科学院南海海洋研究所,中国科学院海洋生物资源可持续利用重点实验室,广东省应用海洋生物学重点实验室,广东广州 510301
2. 中国科学院南海海洋研究所,中国科学院海洋生物资源可持续利用重点实验室,广东省应用海洋生物学重点实验室,广东广州 510301 |
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| 基金项目： | 国家科技支撑计划项目，广东省海洋渔业科技项目，广东省科技计划项目，广东省省院合作计划项目，广西自治区重点科技项目 |
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| 摘 要： | 运用设计出的11个种特异性PCR反向引物,通过与通用正向引物16Sar进行PCR扩增,分别在11种海参中扩增得到大小为269～406 bp的种特异性16S rRNA基因产物,而在非特异种类中扩增不出任何产物,即可有效鉴定这11种海参.此法成功检测出2种冷冻海参替代产品,即用等刺参代替仿刺参,用玉足海参代替棘辐肛参,并且...
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| 关 键 词： | 海参 种特异性PCR 16S rRNA基因 鉴定 |
Species Identification of Sea Cucumber by Species-specific PCR |
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| WEN Jing,HU Chao-qun,ZHANG Lü-ping,FAN Si-gang.Species Identification of Sea Cucumber by Species-specific PCR[J].Fisheries Science,2011,30(12):768-773. |
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| Authors: | WEN Jing HU Chao-qun ZHANG Lü-ping FAN Si-gang |
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| Institution: | 1.Department of Biology,Zhanjiang Normal University,Zhanjiang 524048,China;2.Key Laboratory of Marine Biology,Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China) |
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| Abstract: | The species-specific reverse primers with 269～406 bp species-specific PCR products were obtained and sequenced in the 16S rRNA gene regions in 11 species of sea cucumber by amplification of universal primers 16Sar,without any product of non-specific species.The developed species-specific PCR method was successfully applied to detect 2 substitutes of frozen sea cucumber products,substitution of Isostichopus sp.for Apostichopus japonicus and Holothuria leucospilota for Actinopyga echinites.The developed method authenticated species of 10 unknown dried sea cucumber products.Therefore,this method really provides a simple,rapid and reliable protocol for the accurate identification of sea cucumber species,and also can be very useful for correct labeling of commercial products,conservation of stocks biodiversity,and protection of the consumer rights. |
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| Keywords: | sea cucumber species-specific PCR 16S rRNA gene identification |
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