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| 根癌农杆菌介导gna基因对水稻的转化 | |
| 引用本文： | 张启军,尹福强,王世全,李平,邹江石,吕川根.根癌农杆菌介导gna基因对水稻的转化[J].中国农学通报,2006,22(6):40-40. |
| 作者姓名： | 张启军 尹福强 王世全 李平 邹江石 吕川根 |
| 作者单位： | 1. 江苏省农业科学院粮食作物研究所,江苏,南京,210014;四川农业大学水稻研究所,四川,成都,611130 2. 四川农业大学水稻研究所,四川,成都,611130 3. 四川农业大学水稻研究所,四川,成都,611130;西南作物基因资源与遗传改良教育部重点实验室,四川,雅安,625014 4. 江苏省农业科学院粮食作物研究所,江苏,南京,210014 |
| 基金项目： | 国家863计划项目“超级稻新组合选育”（2003AA212030）和教育部“长江学者和创新团队发展计划”项目“西南作物特异基因资源发掘与利用”（IRT0453）. |
| 摘 要： | 褐飞虱在中国分布很广，近年来已成为南方稻区的主要害虫。大量研究表明,进行抗虫基因分子育种是迅速改良水稻褐飞虱抗性的有效途径。采用农杆菌介导法将来源于大肠杆菌的6-磷酸甘露糖异构酶（PMI）安全选择标记基因和雪花莲凝集素（GNA）抗虫基因构建于同一表达载体上进行遗传转化，获得带有安全标记基因的抗褐飞虱转基因植株。通过PCR扩增、Southern印迹和GUS检测分析表明,PMI标记基因和抗虫GNA基因同时被转入部分植株中并得到了表达，其外缘基因主要以单拷贝的方式插入水稻基因组中。对随机选取的8个转基因单株T0代种子经含潮霉素的培养基培养15d后抗感苗数统计分析表明，绝大多数转基因植株后代符合单基因的遗传分离规律。 |
| 关 键 词： | 水稻 根癌农杆菌 6-磷酸甘露糖异构酶基因 雪花凝集素基因 褐飞虱 |
| 收稿时间： | 2006-01-09 |
| 修稿时间： | 2006-01-092006-02-16 |
Agrobacterium tumefaciens-mediated Transformation of Rice for gna Gene |
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| Zhang Qijun,Yin Fuqiang,Wang Shiquan,Li Ping,Zou Jiangshi,Lv Chuangen.Agrobacterium tumefaciens-mediated Transformation of Rice for gna Gene[J].Chinese Agricultural Science Bulletin,2006,22(6):40-40. | |
| Authors: | Zhang Qijun Yin Fuqiang Wang Shiquan Li Ping Zou Jiangshi Lv Chuangen |
| Institution: | 1.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014; 2.Rice Research Institute of Sichuan Agricultural University, Wenjiang, 611130; 3.Key Laboratory of Southwest Crop Genetic Resources and Improvement (Sichuan Agricultural University |
| Abstract: | Brown plant hopper distributes very wide, and it has been the major rice insects in Southern China. Many studies showed that it was economical and effective method to control insects with transgenic breeding. An expression vector was constructed with a safe marker gene, 6-phosphomannose isomerase (PMI), and an insect-resistant gene, Galanthus nivalis agglutinin(GNA), was used in rice genetic transformation in the present study. Results indicated that the pmi and gna gene have been expressed and transferred into some rice cultivars with PCR analysis, southern blotting and GUS detection, and the gna gene was mainly integrated into rice genome in manner of single copy. Some mature seeds randomly selected from eight transgenic rice plants were cultured 15 days on 1/2 MS medium with 50 mg/L hygromycin, and numbered the resistant and sensitive seedlings. The results indicated that most of the transgenic plants were according with the law of single gene segregation. |
| Keywords: | Rice (Oryza sativa L ) Agrobacterium tumefaciens pmi gna Brown plant hopper |
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