| 黄伞深层液体发酵条件的优化研究 |
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| 引用本文： | 王谦,张俊刚,王士奎,刘会欣,刘玉霞,李志,卢婕.黄伞深层液体发酵条件的优化研究[J].安徽农业科学,2006,34(3):498-499. |
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| 作者姓名： | 王谦 张俊刚 王士奎 刘会欣 刘玉霞 李志 卢婕 |
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| 作者单位： | 河北大学生命科学学院,河北,保定,071002;河北大学生命科学学院,河北,保定,071002;河北省疾病预防控制中心,河北,保定,071000;农业部规划设计研究院,北京,100026 |
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| 基金项目： | 河北省生物工程重点学科项目 |
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| 摘 要： | 采用正交试验及摇瓶培养法对黄伞菌丝深层液体发酵条件进行了优化研究。结果表明,适宜黄伞菌丝深层发酵的培养基组成为:葡萄糖2.0%,豆饼粉2.0%,K2HPO40.05%,MgSO4.7H2O 0.1%,酵母膏0.2%;优化培养条件为:起始pH值6.0,培养温度25℃,摇瓶装量100 ml,摇床转速150 r/min,发酵周期6 d。
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| 关 键 词： | 黄伞 深层液体发酵 优化研究 摇瓶培养法 正交试验 |
| 文章编号： | 0517-6611（2006）03-0498-02 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-11 |
Optimizing Research on Submrged Liquid Fermentation of Pholiota adiposa |
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| WANG Qian et al.Optimizing Research on Submrged Liquid Fermentation of Pholiota adiposa[J].Journal of Anhui Agricultural Sciences,2006,34(3):498-499. |
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| Authors: | WANG Qian |
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| Abstract: | The submerged liquid fermentation conditions of Pholiota adiposa were studied with the method of shake flask culture.The optimal content of culture medium obtained through orthogonal design was as follows: glucose 2.0%,bean cake powder 2.0%,K_2HPO_4 0.05%,MgSO_4·7H_2O 0.1%,yeast and extraction 0.2%.The optimal cultivation conditions of Pholiota adiposa were: original pH 6.0,culture temperature 25?℃,shake flask volume 100 ml,shaker velocity 150?r/min and fermentation time 6?d. |
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| Keywords: | Pholiota adiposa Submerged liquid fermentation Optimizing Research Shake flask culture method |
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