| 三-(2,3-二溴丙基)异氰脲酸酯对斑马鱼胚胎发育的毒性研究 |
| |
| 引用本文： | 邵潘柱,杨迟,林金杏,胡建华.三-(2,3-二溴丙基)异氰脲酸酯对斑马鱼胚胎发育的毒性研究[J].上海交通大学学报(农业科学版),2014(4):76-81. |
| |
| 作者姓名： | 邵潘柱 杨迟 林金杏 胡建华 |
| |
| 作者单位： | [1]东华大学化学化工与生物工程学院,上海201620; [2] 上海实验动物研究中心,上海201203 |
| |
| 基金项目： | 上海市科技发展基金（No.11140902300和No.12140900800） |
| |
| 摘 要： | 为了探究三-(2,3-二溴丙基)异氰脲酸酯(TBC)这一新型含溴阻燃剂对斑马鱼胚胎的毒性效应及潜在的致毒机制,将受精后2h内的斑马鱼胚胎分别暴露于0.2、1、5、25μg/mL的TBC和0.1、0.5、2.5、12.5μg/mL的17β-雌二醇(E2)以及各浓度TBC+2.5μg/mL E2中,分别在24、48、72、120hpf对胚胎进行形态学观察并统计。发现TBC对斑马鱼胚胎72hpf孵化率的抑制作用及120hpf致畸作用呈剂量效应关系,对120hpf胚胎的致死作用较小;E2对斑马鱼胚胎的孵化率亦有抑制作用;在120hpf,TBC和E2对斑马鱼胚胎均有致畸效应但致畸形态有差异;TBC的EC50为1.248μg/mL,E2的EC50为0.968μg/mL及LC50为2.834μg/mL。结果表明,TBC在一定程度上可抑制雌激素对斑马鱼胚胎的毒性效应,呈现潜在的抗雌激素效应的内分泌干扰作用。
|
| 关 键 词： | 斑马鱼 三-(2 3-二溴丙基)异氰脲酸酯 胚胎发育 毒性效应 |
| 收稿时间： | 2013/9/12 0:00:00 |
Study on Developmental Toxicity of the Tris-(2,3-dibromopropyl) Isocyanurate to Zebrafish (Danio rerio)Embryos |
| |
| SHAO Pan-zhu,YANG Chi,LIN Jin-xing and HU Jian-hua.Study on Developmental Toxicity of the Tris-(2,3-dibromopropyl) Isocyanurate to Zebrafish (Danio rerio)Embryos[J].Journal of Shanghai Jiaotong University (Agricultural Science),2014(4):76-81. |
| |
| Authors: | SHAO Pan-zhu YANG Chi LIN Jin-xing and HU Jian-hua |
| |
| Institution: | SHAO Pan-zhu, YANG Chi, L IN J in-xing, HU J ian-hua ( 1. College of Chemistry, Chemical Engineering ＆ Biotechnology, Donghua University, Shanghai 201620, China; 2. Shanghai Lab Animal Research Center, Shanghai 201203, China) |
| |
| Abstract: | In order to explore the toxic effects and potential toxic mechanism of tris-（2,3-dibromopropyl） isocyanurate（TBC） ,a new brominated flame retardant, on the zebrafish embryos. The zebrafish embryos fertilized within 2 hours were exposed by TBC（0.2,1,5, and 25 μg/mL）, E2 （0. 1,0. 5,2.5 and 12. 5 μg/ mL）and various concentrations of TBC＋2.5μg/mL E2. The embryos were observed and counted by a low magnification microscope at 24, 48, 72 and 120 hpf. The toxic effects of TBC on zebrafish embryonic development were evaluated by hatchability, LCso （concentration for 50% of lethal）and ECs0 （concentration for 50% of maximal effect）. The results showed that TBC caused the inhibition of hatchability at 72 hpf and the teratogenic effects at 120 hpf on zebrafish embryos with a dose-dependent manner and the embryo lethal effect of TBC was less at 120 hpf; E2 inhibited the hatchability of the zebrafish embryos at 72 hpf TBC and E2had teratogenic effects on zebrafish embryos with different teratogenic pattern at 120 hpf; the EC50 of TBC was 1. 248 μg/mL, the ECs0 and LC50 of E2 were 0. 968μg/mL and 2. 834 μg/ml, respectively. It indicated that TBC inhibited the toxic effects of estrogen on zebrafish embryos in a certain degree, and showed potential endocrine disruption function with anti-estrogenic effects. |
| |
| Keywords: | zebrafish tris-（2 3-dibromopropyl）isocyanurate embryonic development toxic effects |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《上海交通大学学报(农业科学版)》浏览原始摘要信息 |
| 点击此处可从《上海交通大学学报(农业科学版)》下载免费的PDF全文 |
|
