| 添加物和植物生长调节剂对铁皮石斛离体快速繁殖的影响 |
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| 引用本文： | 殷丽青,孙翊,周音,张永春,朱建军,杨柳燕,李水根,范晓芬.添加物和植物生长调节剂对铁皮石斛离体快速繁殖的影响[J].上海交通大学学报(农业科学版),2014(4):8-13, 23. |
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| 作者姓名： | 殷丽青 孙翊 周音 张永春 朱建军 杨柳燕 李水根 范晓芬 |
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| 作者单位： | 上海市农业科学院林木果树研究所,上海201403 |
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| 基金项目： | 上海市农委种业发展项目（沪农科种字2012第4号） |
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| 摘 要： | 以铁皮石斛未成熟种子为外植体,在离体条件下进行原球茎诱导,类原球茎增殖、分化、生根试验研究。结果表明,以种子萌发直接诱导原球茎的适宜培养基为MS+1.0mg/L BA+0.5mg/L NAA+0.5g/L活性炭(AC)+1.0g/L水解酪蛋白(CH),诱导率达95%以上。在MS+1.0mg/L BA+0.5mg/L KT+0.5mg/L NAA+0.5g/L AC+0.6g/L CH条件下可获得类原球茎最大增殖系数(达18.7),该培养基也适宜于维持类原球茎的增殖保存。类原球茎的最大分化率为93.7%,所需的培养基成分为MS+0.5mg/L BA+0.5mg/L KT+0.2mg/L NAA+0.5g/L AC+0.6g/L CH。试管苗在1/2 MS+0.5mg/L NAA+0.5g/L AC培养基上生根率达100%,且根系健壮发达,移栽后全部成活,长势良好。
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| 关 键 词： | 铁皮石斛 类原球茎 离体快速繁殖 维持 未成熟种子 |
| 收稿时间： | 2014/3/10 0:00:00 |
Effects of Additives and Plant Growth Regulators on In Vitro Propagation in Dendrobium candidum |
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| YIN Li-qing,SUN Yi,ZHOU Yin,ZHANG Yong-chun,ZHU Jian-jun,YANG Liu-yan,LI Shui-gen and FAN Xiao-fen.Effects of Additives and Plant Growth Regulators on In Vitro Propagation in Dendrobium candidum[J].Journal of Shanghai Jiaotong University (Agricultural Science),2014(4):8-13, 23. |
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| Authors: | YIN Li-qing SUN Yi ZHOU Yin ZHANG Yong-chun ZHU Jian-jun YANG Liu-yan LI Shui-gen and FAN Xiao-fen |
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| Institution: | YIN Li-qing , SUN Yi , ZHOU Yin , ZHANG Yong-chun , ZHU J ian-jun , YANG Liu-yan , L I Shui-gen , FAN X iao-f en (Forestry and Pomology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China) |
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| Abstract: | To establish and optimize the technological system for in vitro propagation of Dendrobium candidum, the immatured seeds used as explants were applied to conduct rapid in vitro breeding of D. candidum. The results showed that the optimal medium for seeds germination and the protoeorm induction was MSff- 1. 0 mg/L BA＋0. 5 mg/L NAA＋0. 5 g/L AC （activated carbon）＋1. 0 g/L CH （casein hydrolysate）,and the induction rate was above 95 % The maximum propagation coefficient of protocorm- like-body （PLB） proliferation was 18. 7, which was obtained on the condition of MS＋1.0 mg/L BA＋0. 5 mg/L KT＋0. 5 mg/L NAA＋0. 5g/L AC＋0. 6 g/L CH, and this medium was also suitable for the maintenance of PLBs. After transferred to MS＋0. 5mg/L BA＋0. 5 mg/L KT-F0. 2 mg/L NAA＋0. 5 g/L AC-f-0. 6 g/L CH, PLBs developed into adventitious shoots and the highest differentiation rate was 93.7%. For rooting, adventitious shoots were transplanted on 1/2 MS medium. supplemented with 0. 5 mg/L NAA and 0. 5 g/L AC,the rooting rate was up to 100% and roots developed well. All of the plantlets survived and grew well after transplanted into the soil in the greenhouse. |
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| Keywords: | Dendrobium candidum protocorm-like-bodies （PLBs） in vitro propagation preservation immatured seeds |
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