| ROS对氯化镉诱导的人乳腺癌MCF-7细胞凋亡的影响 |
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| 引用本文： | 周筱轩,陆伟,钟玲盈,钱爱东,周培.ROS对氯化镉诱导的人乳腺癌MCF-7细胞凋亡的影响[J].上海交通大学学报(农业科学版),2014(1):36-42. |
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| 作者姓名： | 周筱轩 陆伟 钟玲盈 钱爱东 周培 |
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| 作者单位： | [1]吉林农业大学动物科技学院,长春130000 [2]农业部都市农业南方重点实验室上海交通大学农业与生物学院,上海200240 |
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| 基金项目： | 上海市自然科学基金青年项目（12ZR1445300）;上海张江国家自主创新示范区专项发展资金重点项目（201209SJ-AO-001）;E海科委项目（11DZ2210800） |
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| 摘 要： | 为研究活性氧(ROS)对氯化镉(CdCl2)诱导的人乳腺癌MCF-7细胞凋亡的影响,本文采用荧光探针DHR检测CdCl2诱导的MCF-7细胞内ROS的变化水平;分别采用MTT法和DNA Ladder法检测CdCl2对细胞的毒性作用和细胞DNA的凋亡情况;通过加入自由基清除剂LNAC抑制细胞内的ROS水平,从而研究ROS对细胞凋亡的影响。结果显示,1.00×10-3 mol/L的CdCl2处理MCF-7细胞6h,细胞内ROS水平为对照组的2.04倍,细胞存活率为26.54%,DNA出现片段化分解。用CdCl2+LNAC复合处理MCF-7细胞6h,细胞内ROS水平为对照组的1.25倍,细胞存活率为61.17%,DNA不出现凋亡条带。因此,由CdCl2所导致的MCF-7细胞凋亡和DNA片段化分解,与细胞内ROS水平的升高有关。
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| 关 键 词： | 人乳腺癌MCF-7细胞 活性氧 N乙酰I 半胱氨酸 细胞凋亡 |
| 收稿时间： | 2013/3/2 0:00:00 |
Effect of ROS on Apoptosis of Human Breast Cancer MCF-7Cells Induced by Cadmium Chlorid |
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| ZHOU Xiao-xuan,LU Wei,ZHONG Ling-ying,QIAN Ai-dong and ZHOU Pei.Effect of ROS on Apoptosis of Human Breast Cancer MCF-7Cells Induced by Cadmium Chlorid[J].Journal of Shanghai Jiaotong University (Agricultural Science),2014(1):36-42. |
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| Authors: | ZHOU Xiao-xuan LU Wei ZHONG Ling-ying QIAN Ai-dong and ZHOU Pei |
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| Institution: | 1. School of Animal Science and Technology,Jilin Agricultural University,Changchun 130000,China; 2. Key Lab of Urban Agriculture (South) of Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China) |
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| Abstract: | To study the role of the reactive oxygen species （ROS） in the cadmium chloride-induced apoptosis of MCF- 7 cells, a cell permeable fluorescent probe dihydrorhodamine （DHR） was used to determine the level of ROS in cadmium chloride challenged MCF-7 cells. Cytotoxic effect was measured by MTT assay. Cell apoptosis was detemained by a DNA Ladder kit. Also the N-Acetyl-DCysteine （LNAC）,a general free radical scavenger,was used to determine the apoptotic effects d ROS on the CdC12 challenged MCF-7 cell. The results showed that, compared with the control group,with 1.00）〈 10 -3 mol/L CdC12 treatment for 6 h,the ROS level was 2. 04 times higher,cell viability was decreased to 26. 54%, and apoptotic fragmentation was detected by gel electrophoresis as a ladder like pattern.However,after LNAC treatment,the ROS level decreased to 1. 25 times. Cell viability increased to 61. 17G ,and it also alleviated the apoptotic DNA fragmentation. These results suggest that cadmium-induced MCF 7 cell apoptosis is due to the increase of intracellular ROS post cadmium challenge. |
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| Keywords: | MCF-7 ROS LNAC apoptosis |
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