| 四君子汤对脾虚大鼠空肠黏膜损伤的修复作用及机制 |
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| 引用本文： | 刘发强,何玉英,李杰峰,张挪威,昝军兰,刘凤华,许剑琴.四君子汤对脾虚大鼠空肠黏膜损伤的修复作用及机制[J].中国兽医杂志,2012,48(5):57-60,103. |
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| 作者姓名： | 刘发强 何玉英 李杰峰 张挪威 昝军兰 刘凤华 许剑琴 |
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| 作者单位： | 1. 中国农业大学动物医学院中兽医实验室,北京海淀,100193
2. 北京农学院动物科技系,北京昌平,102206 |
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| 基金项目： | 高等学校博士学科点专项科研基金，国家自然科学基金 |
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| 摘 要： | 通过检测脾虚大鼠空肠中PCNA和IGF-1的表达,探讨四君子汤对脾虚大鼠空肠黏膜修复的影响。试验分对照组、脾虚组、四君子颗粒治疗组和治疗对照组,每组采取空肠中段作样本;H.E.染色法观察空肠组织结构,免疫组织化学法检测PCNA和IGF-1的表达。脾虚组大鼠空肠绒毛长度、隐窝深度极显著降低(P<0.01);四君子颗粒治疗组大鼠绒毛长度、隐窝深度的显著高于治疗对照组(P<0.05)。脾虚组大鼠空肠中PCNA、IGF-1阳性细胞数极显著增加(P<0.01);四君子颗粒治疗组PCNA、IGF-1阳性细胞个数显著高于治疗对照组(P<0.05)。结果表明,四君子汤可以促进脾虚模型大鼠空肠的修复过程。
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| 关 键 词： | 胰岛素样生长因子-1 PCNA 空肠 脾虚 |
Repair effects and mechanisms of Sijunzi decoction on damage of the Jejunal mucosa in spleen-deficiency rat |
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| LIU Fa-qiang
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HE Yu-ying
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LI Jie-feng
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ZHANG Nuo-wei
,
ZAI Jun-lan
,
LIU Feng-hua
,
XU Jian-qin.Repair effects and mechanisms of Sijunzi decoction on damage of the Jejunal mucosa in spleen-deficiency rat[J].Chinese Journal of Veterinary Medicine,2012,48(5):57-60,103. |
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| Authors: | LIU Fa-qiang HE Yu-ying LI Jie-feng ZHANG Nuo-wei ZAI Jun-lan LIU Feng-hua XU Jian-qin |
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| Institution: | 1(1.China Aagricultural University,Beijing 100193,China; 2.Department of Animal Science and Technology,Beijing College of Agriculture,Beijng,102206,China) |
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| Abstract: | The purpose of this study was to investigate the effect of Sijunzi decoction on repair of the jejunum mucosa of spleen-deficiency rat by detecting the expression of PCNA and IGF-1.This study includes four groups: control group,spleen deficiency group,Sijunzi decoction treatment group and treatment control group.Part of jejunum of each rat was excised as samples;the methods of observation on the structure of jejunum and detection of PCNA and IGF-1 were HE staining and immunohistochemistry,respectively.Villus length and crypt depth of jejunum in spleen deficiency rats decreased significantly(P<0.05);villus length and crypt depth of rats in Sijunzi decoction treatment group was significantly higher than those of rats in control group.The number of both PCNA-positive cells and IGF-1-positive cells in jejunum of spleen deficiency rats increased significantly(P<0.01);the number of both two types of positive cells in spleen deficiency group was all significantly higher than that in control group(P<0.05).These results showed that Sijunzi decoction could accelerate repair of the jejunum mucosa of spleen-deficiency rats. |
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| Keywords: | insulin-like growth factor-1 PCNA jejunum spleen-deficiency |
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