|
|||||
|
|
| 8味中药对奶牛乳房炎病原菌的体外抑菌试验 | |
| 引用本文： | 郭庆,朱伟,易琼,何炳坤,徐宾朋,王鲁.8味中药对奶牛乳房炎病原菌的体外抑菌试验[J].中国兽医杂志,2012,48(5):63-65. |
| 作者姓名： | 郭庆 朱伟 易琼 何炳坤 徐宾朋 王鲁 |
| 作者单位： | 1. 贵州大学贵州省生化工程中心,贵州贵阳550025;安徽科宝生物工程有限公司,安徽淮北235025 2. 贵州大学动物科学学院,贵州贵阳,550025 3. 北京生泰尔生物科技有限公司,北京昌平,102206 4. 安徽科宝生物工程有限公司,安徽淮北,235025 5. 贵州大学贵州省生化工程中心,贵州贵阳,550025 |
| 基金项目： | 贵州省科技支撑计划项目 |
| 摘 要： | 选用金银花、连翘、蒲公英等8味中药分别采用乙醇回流法进行提取,用试管二倍稀释法测定来自奶牛乳房炎的3种主要致病菌(金黄色葡萄球菌、无乳链球菌、停乳链球菌)的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果显示,以金银花和连翘对金黄色葡萄球菌抑菌效果最强,MIC值均为15.63mg/mL,其MBC值为31.25mg/mL和62.5mg/mL;芙蓉花对大肠杆菌和无乳链球菌的效果最强,MIC值为31.25mg/mL和15.63mg/mL,MBC值为62.5mg/mL。本试验结果,对临床用药选取合适的中药有直接的指导意义,同时为体内用药的浓度提供参考依据。 |
| 关 键 词： | 中药 奶牛乳房炎 致病菌 MIC MBC |
Antibacterial effects of eight Chinese herbal medicine on the pathogens of dairy cow mastitis in vitro |
|
| GUO Qing , ZHU Wei , YI Qiong , HE Bing-kun , XU Bin-peng , WANG Lu.Antibacterial effects of eight Chinese herbal medicine on the pathogens of dairy cow mastitis in vitro[J].Chinese Journal of Veterinary Medicine,2012,48(5):63-65. | |
| Authors: | GUO Qing ZHU Wei YI Qiong HE Bing-kun XU Bin-peng WANG Lu |
| Institution: | 1(1.Research Center of Biochemistry Engineering of Guizhou Province,Guizhou University,Guiyang 550025,China; 2.Anhui Chem-Bright Bioengineering Co.,Ltd,Huaibei 235025,China;3.College of Animal Science, Guizhou University,Guiyang 550025,China;4.Beijing Centre Bio-Tech Co.,Ltd,Beijing 102206,China) |
| Abstract: | Experiments have been carried out to test the minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of the alcohol extracts of eight kinds of Chinese herbal medicines(such as honeysuckle,forsythia,dandelion,etc) on 3 strains of pathogenic bacteria of bovine mastitis,which are staphylococcus aureus,E.coli,and streptococcus agalactiae with double dilution method in vitro.The results showed that honeysuckle and forsythia had obvious antibacterial effect on staphylococcus aureus,and the MIC value of both medicine was 15.63 mg/mL,and the MBC value was 31.25 mg/mL and 62.5 mg/mL,respectively.Hibiscus flower had better antibacterial effect on E.coli and Streptococcus agalactiae,and the MIC value was 31.25 mg/mL and 15.63 mg/mL,respectively.The MBC value of both was 62.5 mg/mL.The data here could give us some clues for the selection of clinical medicines and provide us a reference for the concentration of Chinese herbal medicine used in vivo. |
| Keywords: | Chinese herbal medicines dairy cow mastitis pathogenic bacterium MIC MBC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |