Relationships in pineapple by random amplified polymorphic DNA (RAPD) analysis |
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| Authors: | S Sripaoraya N W Blackhall R Marchant J B Power K C Lowe M R Davey |
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| Institution: | Plant Science Division, School of Biosciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK. E-mail:;School of Life and Environmental Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK |
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| Abstract: | Random amplified polymorphic DNA (RAPD) analysis was applied to eight commercial cultivars of pineapple, two intergroup hybrids and two wild species. Morphologically, pineapple is divided into the Cayenne, Queen, Spanish, Maipure and Abacaxi groups. Members of the first three groups have been analysed in this study. The cultivars ‘Tradsithong’, ‘Phuket’, ‘Sawee’ and ‘Tainan’, with spiny leaves, form the Queen group. In ‘Pattavia’, ‘Nanglae’ and ‘Petburi no. 2’ (Cayenne group), spines are confined to the leaf tips. ‘Intrachitdang’ is normally placed in the Spanish group, which is morphologically similar to the Queen group, but with inferior quality fruit. DNA amplification products were compared from 16 arbitrary 10‐mer primers from which a dendrogram was constructed. The results confirmed morphological classifications for seven of the eight commercial cultivars, with the Queen and Cayenne groups as separate clusters. However, the cv. ‘Intrachitdang’ was more closely related to the Cayenne group. Two hybrids from reciprocal Cayenne × Queen group crosses, were more closely allied to the Queen group. The two wild species were outside the groups. RAPD analysis can be exploited to investigate relationships within pineapple germplasm. |
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| Keywords: | Ananas comosus breeding strategies commercial production genetic relationships Jaccard's coefficient RAPD |
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