Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina |
| |
| Authors: | MA Vissani ML Becerra C Olguín Perglione MS Tordoya S Mio M Barrandeguy |
| |
| Institution: | aInstituto de Virología, CICVyA, INTA. Las Cabañas y los Reseros s/n, (1712) Castelar, Buenos Aires, Argentina |
| |
| Abstract: | Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N752/D752) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In order to estimate the prevalence of the EHV-1 neuropathogenic genotype in our country, we analyzed the ORF30 genome region of Argentinean EHV-1 isolates. The study was carried out by real time allelic discrimination PCR in 90 equine EHV-1-positive samples, being 89 from 54 cases of abortion outbreaks (two of which were in association with neurological disease) and one from the respiratory tract of a healthy horse in training. Our results indicate that 7% (4/54) of the abortion outbreaks studied were induced by the neuropathogenic (G2254) genotype of EHV-1 and 50% (2/4) of them were associated with simultaneous neurological disease. This information emphasizes the necessity to extreme the hygienic and preventive measures to diminish EHV-1 infections and consequently reduce the risk of epizootic neurological disease as has been recently observed in other countries. |
| |
| Keywords: | Equid Herpesvirus type 1 Equid herpesvirus myeloencephalopathy EHV-1 neuropathogenic genotype EHV-1 abortion outbreaks |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
