| Establishment of genetic transformation system via Agrobacterium in tall fescue cultivar |
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| 基金项目： | Foundation project： This paper was supported by Zhejiang Provincial Science and Technology Plan of China （Grant No. 2003C30053） and Zhejiang Provincial Natural Science Foundation of China （Grant No.Y504076）. |
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| 摘 要： |
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| 关 键 词： | 高羊茅 农杆菌 转化 uidA基因 |
| 文章编号： | 1007-662X（2006）03-0238-05 |
| 收稿时间： | 2006-01-16 |
| 修稿时间： | 2006-03-12 |
Establishment of genetic transformation system via Agrobacterium in tall fescue cultivar |
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| Authors: | Hai-feng Qian Shaukat Ali Liang Hong Hao Xu |
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| Institution: | (1) College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, China;(2) National Agricultural Research Centre, Park Road, Islamabad, Pakistan |
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| Abstract: | Tall fescue (Festuca arundinacea Schreb.) is a cool-season turfgrass used on fairways in golf courses. The object of this study was to develop a more efficient,
reliable, and repeatable approach in transforming the grass using Agrobacterium (EHA105), where β-glucuronidase gene (uidA) was used as a reporter and hygromycin phosphotransferase gene (hyg) as a selectable marker. An effective expression of transgene
was observed in transforming 2-month-old calli derived from mature seeds (cv. Bingo) cultured on MS medium supplemented with
9 mg·L−1 2, 4-D. A two-step solid medium selection with increasing hygromycin concentration (from 30 to 50 mg·L−1) was used to obtain resistant calli. Transgenic plants have been produced from many independent transformed calli. The presence
of functional β-glucuronidase gene (uidA) was detected in hygromycin-resistant calli. Transgenic plants were regenerated and PCR and Southern blot confirmed transgene
integration in the tall fescue genome.
Biography: QIAN Hai-feng (1973–), male, Ph.D., associate professor in Zhejiang University of Technology. |
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| Keywords: | Tall Fescue Agrobacterium tumefaciens Transformation β -glucuronidase gene (uidA) |
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