| 国产IBV疫苗株膜蛋白基因的亲缘关系研究 |
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| 引用本文： | 廖明,曹伟胜,任涛,罗开健,辛朝安.国产IBV疫苗株膜蛋白基因的亲缘关系研究[J].中国预防兽医学报,2002,24(3):185-189. |
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| 作者姓名： | 廖明 曹伟胜 任涛 罗开健 辛朝安 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 基金项目： | 国家自然科学基金重大项目 (398932 90_2 ) |
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| 摘 要： | 利用自行设计的引物Wx和Ws，通过RT－PCR方法分别扩增出IBVD41株、H120GD株、H120SH株、H520GD株4个国产疫苗株和标准强素M41－E4株完整的M基因cDNA，然后将其分别克隆到pGMET－Easy或pMD18－T载体中。测序结果发现：这5个IBV毒株的M基因均为678bp，分别编码由225个氨基酸残基组成的多肽。序列分析表明：D41株、H120GD株和H120SH株M基因之间的核苷酸序列及推导氨基酸序列同源性均为100％，它们在系统发生进化树中处同一分支；而H52GD株和国内的M41－E4株和国外的M41株M基因之间的核苷酸及推导氨基酸序列同源性分别为99．9％和99．6％，它们之间的亲缘关系最近，在系统发生进化树上处另一个分支。
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| 关 键 词： | 鸡传染性支气管炎 序列分析 IBV疫苗株 膜蛋白基因 亲缘关系 毒株 RNA病毒 |
| 文章编号： | 1008-0589(2002)03-0185-05 |
| 修稿时间： | 2001年11月20 |
Genetic Relationship of Membrane Gene of Domestic IBV Va ccine Strains |
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| LIAO Ming,CAO Wei_sheng,REN Tao,LUO Kai_jian,XIN Chao_an.Genetic Relationship of Membrane Gene of Domestic IBV Va ccine Strains[J].Chinese Journal of Preventive Veterinary Medicine,2002,24(3):185-189. |
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| Authors: | LIAO Ming CAO Wei_sheng REN Tao LUO Kai_jian XIN Chao_an |
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| Abstract: | RT_PCR was employed to amplify the cDNAs of membrane protein gene(M gene)of IBV strains D41,H120GD,H120SH,H52GD and M41_E4 with a pair of specific oligonucleotide primers(Wx/Ws)and the cDNAs were cloned into the T_T windows of plasmid pGEM_T Easy and pMD 18_T respectively.The inserts were sequenced and the results showed that the full length sequences of M gene of strains D41,H120GD,H120SH,H52GD and M41_E4 consisted of 678 nucleotides (nt)respectively,coding for predicated polypeptides of 225 amino acid residues.Analyses of the M gene nucleotide sequences and deduced amino acid sequences suggested;strains D41,H120GD and H120SH shared 100% identity with each other and they occurred in the same branch of the phylogenetic tree of M gene,While there was 99.9% nucleotide sequence homology and 99.6% deduced amino acid sequence homology between H52GD vaccine strain and domestic virulent M41_E4 reference strain and foreign M41 strain,and they were situated in another branch because of the closest genetic relationship. |
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| Keywords: | Infectious bronchitis virus Vaccine strain Membrane(M) gene Sequence analysis |
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