| 鲤鱼精子染色方法学研究 |
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| 引用本文： | 王建福,李洁,邵淑娟,张勤,张兆旺.鲤鱼精子染色方法学研究[J].甘肃农业大学学报,2013,48(3). |
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| 作者姓名： | 王建福 李洁 邵淑娟 张勤 张兆旺 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃兰州,730070 |
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| 基金项目： | 甘肃农业大学动物科学技术学院2010年青年教师科研基金 |
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| 摘 要： | 用龙胆紫、碱性复红、吉姆萨、甲基绿、苏木精-伊红Y、伊红Y-乙醇、伊红Y-水、伊红Y-苯胺黑等染液对鲤鱼精子进行染色,筛选适宜的鲤鱼精子染色方法.结果表明:采用不干燥固定直接伊红Y-乙醇压片染色的效果最好,染色清晰,精子头尾结构完整;自然干燥固定后再染色,可使鲤鱼精子头膜破裂细胞质漏出,致使精子染色形态模糊不清;4%的甲醛固定后再经自然干燥染色,可以有效避免鲤鱼精子头膜破裂;用1%的NaCl稀释的精液染色后,可清楚地观察到精子形态并区分出畸形精子,用水稀释的精液染色后可观察精子尾膜的完整性;体外活体伊红Y-苯胺黑染色法可以观察出精子头膜的完整性以及精子的损伤程度.
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| 关 键 词： | 鲤鱼精子 干燥固定 染色 形态观察 |
A study on different staining methods to Cyprinus carpio spermatozoon |
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| WANG Jian-fu
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LI Jie
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SHAO Shu-juan
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ZHANG Qin
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ZHANG Zhao-wang.A study on different staining methods to Cyprinus carpio spermatozoon[J].Journal of Gansu Agricultural University,2013,48(3). |
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| Authors: | WANG Jian-fu LI Jie SHAO Shu-juan ZHANG Qin ZHANG Zhao-wang |
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| Abstract: | To select a appropriate method of staining Cyprinus carpio spermatozoon,gentian violet,basic fuchsin,Giemsa,methyl green,hematoxylin eosin Y,eosin Y-ethanol,eosin Y-water,eosin Y-aniline black dye liquors were applied.The results showed that tabletting the spermatozoon with eosin Y before drying was the best method,the staining was clear,sperm was integrity.Staining after natural drying could make Cyprinus carpio spermatozoon′s head membrane rupture cytoplasmic leakage,resulting in sperm staining patterns fuzzy and incomplete.4% formaldehyde fixed before natural drying could avoid carp sperm head membrane rupture.With 1% NaCl diluted semen staining could be clearly observed that the sperm morphology and distinguish abnormal sperm,diluted semen staining could be observed in sperm tail membrane integrity.A supravital eosin Y-aniline dyeing method could be observed head membrane integrity and damage degree of the sperm. |
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| Keywords: | Cyprinus carpio spermatozoon drying fixation staining morphological observation |
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