| 花生中蔗糖合成酶基因AhSuSy在花生中的组织表达及对非生物胁迫响应研究 |
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| 引用本文： | 陈娜,胡冬青,王道远,潘丽娟,迟晓元,陈明娜,王通,王冕,杨珍,禹山林.花生中蔗糖合成酶基因AhSuSy在花生中的组织表达及对非生物胁迫响应研究[J].花生学报,2013(4):25-32. |
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| 作者姓名： | 陈娜 胡冬青 王道远 潘丽娟 迟晓元 陈明娜 王通 王冕 杨珍 禹山林 |
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| 作者单位： | [1]山东省花生研究所,山东青岛266100 [2]农业部油料作物生物学与遗传育种重点实验室／中国农业科学院油料作物研究所,湖北武汉430062 [3]青岛市出入境检验检疫局,山东青岛266001 [4]章丘农业局,山东济南250200 |
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| 基金项目： | 国家产业体系（CARS-14）;山东省自然基金（ZR2009DQ004;ZR2011CQ036;ZR2012CQ031）;国家自然基金（31000728;31100205;31200211）;农业部油料作物生物学与遗传育种重点实验室开放课题基金（2014010）;山东省优秀中青年科学家科研奖励基金（BS2010NY023）;青岛市科技计划（11-2-4-9-（3）-jch;11-2-3-26-nsh;12-1-4-11-（2）-jch）;青岛市成果培育项目（12-6-4-7-chg） |
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| 摘 要： | 低温、高盐和干旱胁迫严重影响植物的生长和产量。本研究以花生品种花育33号为实验材料，根据cDNA文库中已知的蔗糖合成酶基因AhSuSy全长序列设计引物，通过RT—PCR克隆到该基因。通过荧光定量PCR分析了该基因在花生各组织中的表达及在低温、高盐等非生物胁迫下的表达。结果显示，该基因为组成型表达基因，在叶片和根中表达量较高，在花中表达量最低；AhSuSy基因在花生的叶片和根中对低温均没有明显响应，但在花生根中受高盐胁迫和干旱胁迫明显诱导，说明该基因可能参与了花生对高盐和干旱胁迫的适应性调控；AhSuSy在花生根中受ABA的明显诱导，说明该基因对花生非生物胁迫的调控可能是以依赖ABA的方式进行的。
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| 关 键 词： | 花生 蔗糖合成酶 表达分析 非生物胁迫 |
Expression Analysis of the Sucrose Synthase Gene AhSuSy in Different Tissues and under Abiotic Stresses in Peanut |
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| CHEN Na,HU Dong-qing,WANG Dao-yuan,PAN Li-juan,CHI Xiao-yuan,CHEN Ming-na,WANG Tong,WANG Mian,YANG Zhen,YU Shan-lin.Expression Analysis of the Sucrose Synthase Gene AhSuSy in Different Tissues and under Abiotic Stresses in Peanut[J].Journal of Peanut Science,2013(4):25-32. |
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| Authors: | CHEN Na HU Dong-qing WANG Dao-yuan PAN Li-juan CHI Xiao-yuan CHEN Ming-na WANG Tong WANG Mian YANG Zhen YU Shan-lin |
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| Institution: | 1. Shandong Peanut Research Institute, Qingdao 266100, China; 2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of Agri. Sci. , Wuhan 430062, China; 3. Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266001, China; 4. Zhangqiu Agriculture Bureau, Jinan, 250200, China) |
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| Abstract: | Plant growth and yield are strongly influenced by abiotic stresses such as drought, salt and cold. In this article, we cloned the full length of sucrose synthase gene AhSuSy from peanut （Arachis hypogaea L. cultivar Huayu33） through RT-PCR method. The expression pattern of Ah- SuSy in different peanut tissues and under various abiotic stresses were detected with realtime RT- PCR. The results showed that AhSuSy was expressed in all the six tissues studied, with higher expression level in the leaves and roots and the lowest level in the flowers. The expression of AhSuSy had no obvious change under cold stress in peanut leaves and roots. However, AhSuSy was induced distinctly in peanut roots during salt and drought conditions. These results suggested that AhSuSy may participate in the salt and drought stress regulation o{ peanut. The expression of AhSuSy was also induced by exogenous ABA, which indicated that AhSuSy may regulate peanut abiotic stress resist ance through ABA-dependent way. |
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| Keywords: | peanut sucrose synthase expression analysis abiotic stresses |
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