Genetics and Molecular Mapping of Stripe Rust Resistance Gene YrShan515in Chinese Wheat Cultivar Shan 515 |
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| Authors: | ZHANG Shu-ying XU Zhong-qing WANG Rui LI Qiang YAO Qiang JING Jin-xue |
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| Institution: | 1. Shaanxi Key Laboratory of Molecular Biology for Agriculture/College of Plant Protection, Northwest A&F University, Yangling 712100, P.R. China
2. Shaanxi Key Laboratory of Molecular Biology for Agriculture/College of Plant Protection, Northwest A&F University, Yangling 712100, P.R. China;Qinghai Academy of Agriculture and Forestry, Qinghai 810000, P.R.China |
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| Abstract: | Stripe rust is one of the most important wheat diseases worldwide. To identify new resistance genes is significant in wheat breeding. In this study, stripe rust resistance of a Chinese cultivar Shah 515 was tested with Chinese predominant races of P. striiformis f. sp. tritici in the seedling stage, and genetic analysis and simple sequence repeats (SSR) technique were used to identify the inheritance model of seedling stripe rust resistance in cultivar Shan 515 and to mark the sites of resistance gene(s) on chromosome. The genetic analysis indicated that the resistance of Shan 515 against Su 11-4 was conferred by a single dominant gene, which was temporarily designated as YrShan515. Using bulked segregant analysis (BSA) and SSR markers, 12 SSR markers (Xwmc335, Xwmc696, Xwmc476, Xbarc267, Xgwm333, Xwmc653, Xwmc396,Xgwm213, Xgwm112, Xgwm274, Xcfd22, Xgwm131, and Xwmc517) located on wheat chromosome 7BL were linked to YrShan515 with genetic distance ranging from 3 to 24 eM. Based on the previously published genetic map and Chinese Spring nulli-tetrasomic analysis, YrShan515 was located on wheat chromosome 7BL. Polymorphism of wheat cuitivars collected from Huanghuai wheat grown regions were screened with two markers, Xwmc653 and Xbarc267, and all of these wheat cultivars tested did not present the polymorphic bands as Shan 515 did. Therefore, it suggested that YrShan515 might be a allele of the available yellow rust resistance gene. The mapping of the new resistance gene in Shan 515 is useful for wheat breeding and diversification of resistance genes against stripe rust in commercial wheat cultivars in China. |
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| Keywords: | wheat cultivar Puccinia striiformis f sp Tritici resistance genes molecular mapping |
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