| 培养条件对胸膜肺炎放线杆菌生长的影响 |
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| 引用本文： | 赵萍,逯忠新,储岳峰,高鹏程,贺英,石琴.培养条件对胸膜肺炎放线杆菌生长的影响[J].中国畜牧兽医,2008,35(4):80-82. |
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| 作者姓名： | 赵萍 逯忠新 储岳峰 高鹏程 贺英 石琴 |
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| 作者单位： | 中国农业科学院兰州兽医研究所 家畜疫病病原生物学国家重点实验室 农业部畜禽病毒学重点开放实验室，兰州 730046 |
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| 基金项目： | 国家重点新产品(2004ED860008) |
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| 摘 要： | 通过对胸膜肺炎放线杆菌在液体培养基中不同培养条件下（静止培养、摇床培养和发酵培养）的比较试验得出静止培养细菌最高浓度为1×109个/ml，所需时间为18 h；摇床培养细菌最高浓度为3.7×109个/ml，所需时间为13 h；发酵罐通氧气（20%）培养细菌最高浓度为4.5×109个/ml，所需时间为4 h；发酵罐不通氧气培养细菌最高浓度为2.0×109个/ml，所需时间为4 h。由此得出胸膜肺炎放线杆菌在液体培养基中随着培养条件的不同其菌液浓度以及完成对数期的时间也不同，发酵（通氧气）培养菌液浓度高于其它培养方式且所需时间最短，这一结果为胸膜肺炎放线杆菌抗原的大批量生产奠定了很好的基础。
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| 关 键 词： | 培养条件 胸膜肺炎放线杆菌 |
| 文章编号： | 1671-7236(2008)04-0080-02 |
| 修稿时间： | 2007年11月6日 |
Comparisons of Three Cultivation Conditions Affecting on the Growth of Actinacillus Pleuropeumoniae |
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| ZHAO Ping,LU Zhong-xin,CHU Yue-feng,GAO Peng-cheng,HE Ying,SHI Qin.Comparisons of Three Cultivation Conditions Affecting on the Growth of Actinacillus Pleuropeumoniae[J].China Animal Husbandry & Veterinary Medicine,2008,35(4):80-82. |
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| Authors: | ZHAO Ping LU Zhong-xin CHU Yue-feng GAO Peng-cheng HE Ying SHI Qin |
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| Institution: | Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China |
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| Abstract: | The concertration and the time for logarithmic phase of actinacillus pleuropeumoniae are different due to the difference of cultivationd condition in liquid medium. In this report ,three cultivation methods of stilness, shake and fermentation were used and compared.The data revealed that stillness the highest concertration was 1×109 and time was eighteen hours, shake the highest concertration was 3.7×109 and time was thirteen hours, fermentation the highest concertration was 4.5×109 and time was four hours.Fermentation the highest concertration higher than other two methods and the cultivated process was shortern apparently.Therefore, a foundation was built for producation Actinacillus Pleuropeumoniae antigen massively. |
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| Keywords: | cultivation condition actinacillus pleuropeumoniae |
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