| 茄子单性结实基因的遗传分析及AFLP分子标记 |
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| 引用本文： | 刘富中,万翔,陈钰辉,连勇,宋明.茄子单性结实基因的遗传分析及AFLP分子标记[J].园艺学报,2008,35(9):1305-1309. |
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| 作者姓名： | 刘富中 万翔 陈钰辉 连勇 宋明 |
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| 作者单位： | （中国农业科学院蔬菜花卉研究所，北京 100081;四川资阳农业局，四川资阳641300；西南大学园艺园林学院，重庆400716） |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，中央级公益性科研院所基本科研业务费专项，农业部重点实验室基金 |
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| 摘 要： | 以茄子单性结实自交系D-10和非单性结实自交系03-2为试验材料，研究了单性结实基因的遗传特性及其AFLP标记。对杂交后代F1、F2、BC1单性结实性表现型分离比例的分析表明，茄子D-10的单性结实性由单显性核基因控制，其基因用符号Pat表示。采用AFLP分析技术和改良BAS法，通过512对E/M引物组合的筛选，获得1个与茄子单性结实基因紧密连锁的AFLP标记E75/M53-70，该标记与单性结实基因间的遗传图距为15.38 cM，可用于茄子单性结实性的鉴定和单性结实分子标记辅助育种，加速茄子单性结实基因的转育和利用。
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| 关 键 词： | 茄子 单性结实基因 遗传规律 AFLP标记 |
| 收稿时间： | 2008-6-23 |
| 修稿时间： | 2008-8-8 |
Inheritance of the Eggplant Parthenocarpy and AFLP Molecular Marker |
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| LIU Fu-zhong,WAN Xiang,CHEN Yu-hui,LIAN Yong,SONG Ming.Inheritance of the Eggplant Parthenocarpy and AFLP Molecular Marker[J].Acta Horticulturae Sinica,2008,35(9):1305-1309. |
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| Authors: | LIU Fu-zhong WAN Xiang CHEN Yu-hui LIAN Yong SONG Ming |
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| Institution: | (;1.Institute of Vegetables & Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China；;2.the Ziyang County office of Agricultural, Ziyang ,Sichuan 641300, China；;3.Department of HorticuLture, Southwest University, Chongqing 400716，China) |
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| Abstract: | Emphytic character and AFLP marker of eggplant parthenocarpic gene were studied by using materials of parthenocarpic selfing line D-10 and un-parthenocarpic selfing line 03-2. Analysing the separated rate of the parthenocarpic phenotype in F1,F2, and BC1, we found that parthenocarpic gene of D-10 was controlled by single dominant nucleus gene and named as Pat. Using AFLP and BSA techniques with 512 pairs of E/M primers screening, we obtained one AFLP marker E75/M53-70 that linked with parthenocarpic gene.The genetic distance was 15.38 cM，which could be used as parthenocarpic identification and molecular marker-assisted selection. |
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| Keywords: | eggplant parthenocarpic gene genetic regulation AFLP molecular marker |
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