Slow-growth in vitro conservation of potato germplasm at normal propagation temperature |
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| Authors: | J Gopal Anjali Chamail Debabrata Sarkar |
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| Institution: | (1) Division of Crop Improvement, Central Potato Research Institute, Shimla-171 001 Himachal Pradesh, India |
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| Abstract: | Summary In-vitro conservation of microplants at 24±1 °C was investigated in six potato genotypes belonging to different maturity groups.
Growth was controlled by using different concentrations of sucrose (20, 40 and 60 g 1−1) alone or in combination with either mannitol (20 and 40 g 1−1) or sorbitol (20 and 40 g 1−1) in Murashige and Skoog medium. Maximum microplant survival (55.5–77.8%) after 12 months of storage was on medium supplemented
with 20 g 1−1 sucrose plus 40 g 1−1 sorbitol. Microplants so conserved were in good to very good condition, without phenotypic abnormalities and had enough nodes
for sub-culturing. Microplant survival and microplant condition were closely associated with each other but not with root
growth. The success of conservation was unaffected by maturity group of the genotypes. This conservation approach at 24±1
°C can be an effective alternative to low temperature (6–8 °C) storage, especially in tropical and sub-tropical conditions,
where the ambient temperatures in summer can reach 45–50°C. |
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| Keywords: | optimal conditions osmotic stress microplant survival root development Solarium tuberosum L |
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