| RP-HPLC检测复方注射剂中伊维菌素和吡喹酮的含量 |
| |
| 引用本文： | 林红.RP-HPLC检测复方注射剂中伊维菌素和吡喹酮的含量[J].中国兽药杂志,2011,45(7):30-31. |
| |
| 作者姓名： | 林红 |
| |
| 作者单位： | 齐鲁动物保健品有限公司山东省化学兽药工程技术研究中心,济南,250100 |
| |
| 摘 要： | 为了用高效液相色谱法同时测定复方注射剂中伊维菌素和吡喹酮的含量。采用Hyper-C lone 5u C18柱（250 mm×4.60 mm,5μm）,以乙腈-甲醇-水（53∶35∶12）为流动相,流速为1.0mL/m in,检测波长254 nm,柱温30℃的液相检测方法。结果表明,在此色谱条件下,吡喹酮和伊维菌素的分离良好,吡喹酮和伊维菌素分别在30~500μg/mL和5~40μg/mL浓度范围内峰面积与浓度呈良好的线性关系（r2〉0.99）。按外标法以峰面积计算进行测定,吡喹酮和伊维菌素的平均回收率分别为98.93%和95.89%,RSD分别为0.33%和1.58%（n=9）。说明该方法简便、快速、准确,适用于复方吡喹酮注射液中吡喹酮和伊维菌素含量的测定。
|
| 关 键 词： | 高效液相色谱法 吡喹酮 伊维菌素 |
| 收稿时间： | 1/16/2011 4:25:38 PM |
| 修稿时间： | 2011/5/27 0:00:00 |
RP-HPLC assay for praziquantel and ivermectin in an injectable compound |
| |
| linhong.RP-HPLC assay for praziquantel and ivermectin in an injectable compound[J].Chinese Journal of Veterinary Drug,2011,45(7):30-31. |
| |
| Authors: | linhong |
| |
| Institution: | Qilu Animal Health Products Co.Ltd |
| |
| Abstract: | To detect the concentration of praziquantel and ivermectin in an injectable compound.The HPLC method was established,using a HyperClone 5u C18 column（250 mm×4.60 mm,5 μm）and acetonitrile-methanol-water（53∶35∶12）as the mobile phase at a flow rate of 1 mL per minute,with UV detector at 254 nm.The results indicated that calibration curves for praziquantel and ivermectin were linear over the range of 30～500 μg/mL and 5～40 μg/mL（r20.99）,respectively.The mean recoveries of praziquantel and ivermectin were 98.93% and 95.89%,and RSD were 0.33% and 1.58%（n=9）,respectively.In conclusion,the method is simple,rapid and reliable for the determination of praziquantel and ivermectin in compound praziquantel injection. |
| |
| Keywords: | high-performance liquid chromatography praziquantel ivermectin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 |
| 点击此处可从《中国兽药杂志》下载免费的PDF全文 |
|
