Immunomodulatory Properties of the Protein Fraction from Phorphyra columbina |
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| Authors: | Raúl E Cian Rocío López-Posadas Silvina R Drago Fermín Sánchez de Medina Olga Martínez-Augustin |
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| Institution: | Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral , 1° de Mayo 3250, (3000) Santa Fe, Argentina. |
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| Abstract: | The phycobiliproteins from Rhodophyta , R-phycoerythrin (R-PE) and C-phycocyanin (C-PC), have been shown to exert immunomodulatory effects. This study evaluated the effects of a Phorphyra columbina protein fraction (PF) and R-PE and C-PC on rat primary splenocytes, macrophages, and T-lymphocytes in vitro. PF featured various protein species, including R-PE and C-PC. PF showed mitogenic effects on rat splenocytes and was nontoxic to cells except at 1 g L(-1) protein. IL-10 secretion was enhanced by PF in rat splenocytes, macrophages, and especially T-lymphocytes, whereas it was markedly diminished by R-PE and C-PC. The production of pro-inflammatory cytokines by macrophages was inhibited. The effect of PF on IL-10 was evoked by JNK/p38 MAPK and NF-κB-dependent pathways in macrophages and T-lymphocytes. It was concluded that PF has immunomodulatory effects on macrophages and lymphocytes that appear to be predominantly anti-inflammatory via up-regulated IL-10 production and cannot be accounted for by R-PE and C-PC. |
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