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Transient expression vectors for functional genomics,quantification of promoter activity and RNA silencing in plants
Authors:Email author" target="_blank">Roger?P?HellensEmail author  Andrew?C?Allan  Ellen?N?Friel  Karen?Bolitho  Karryn?Grafton  Matthew?D?Templeton  Sakuntala?Karunairetnam  Andrew?P?Gleave  William?A?Laing
Institution:(1) HortResearch, Mt Albert Research Centre, Private Bag, 92169, Auckland, New Zealand
Abstract:

Background  

We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformation such as transformation selection genes.
Keywords:
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