| 高寒牧草内生细菌分离培养条件的优化 |
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| 引用本文： | 满百膺,陈秀蓉,李振东.高寒牧草内生细菌分离培养条件的优化[J].草原与草坪,2008(5):27-30. |
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| 作者姓名： | 满百膺 陈秀蓉 李振东 |
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| 作者单位： | 甘肃农业大学,草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃,兰州,730070 |
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| 基金项目： | 甘肃农业大学草业学院草业科学国家级重点学科学术骨干科研项目，甘肃农业大学草业学院草业科学国家级重点学科学术骨干科研项目 |
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| 摘 要： | 通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0．19／6SDS浸泡15min、3％NaClO浸泡3min、0．1％升汞浸泡10min、75％酒精1～2min处理后（各步间均用无菌水冲洗3～4次），研磨并稀释至10^-3，涂布于TSA培养基上，置28℃恒温条件下培养5—7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。用此方法，已从5种牧草不同组织器官（根、茎、叶、花）中分离获得大小、形态、颜色各异的内生细菌315株。
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| 关 键 词： | 高寒牧草 内生细菌 分离条件 优化 |
Optimization for isolation and culture conditions of endophytic bacteria in grasses in alpine area |
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| MAN Bai-ying,CHEN Xiu-rong,LI Zhen-dong.Optimization for isolation and culture conditions of endophytic bacteria in grasses in alpine area[J].Grassland and Turf,2008(5):27-30. |
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| Authors: | MAN Bai-ying CHEN Xiu-rong LI Zhen-dong |
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| Institution: | College of Pratacultural Science;Gansu Agricultural University;Key Laboratory of Grassland Ecosystem;Ministry of Education;Sion-U.S.Centers for Grazingland Ecosystem Sustainability;Lanzhou 730070;China |
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| Abstract: | The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study.The different tissues of grasses were immersed in 0.1% of SDS for 15 min,3% of NaClO for 3 min,0.1% of mercuric chloride for 10 min,75% of alcohol for 1 to 2 min.The endophytic bacteria were isolated after aseptic grinding and diluted to 10-3 and using TSA culture medium for 5 to 7 days under 28 ℃.315 strains of endophytic bacteria with different size,shape and color in different tissues of five grasses were obtained |
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| Keywords: | alpine pasture endophytic bacteria isolation condition optimization |
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