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| 草莓悬浮细胞原生质体培养再生愈伤组织 | |
| 引用本文： | 周春江,李卫东,葛会波.草莓悬浮细胞原生质体培养再生愈伤组织[J].果树学报,2003,20(4):319-321. |
| 作者姓名： | 周春江 李卫东 葛会波 |
| 作者单位： | 1. 河北师范大学生命科学学院,河北,石家庄,050016;河北农业大学园艺系,河北,保定,071001 2. 河北农业大学山区研究所,河北,保定,071001 3. 河北农业大学园艺系,河北,保定,071001 |
| 摘 要： | 以草毒品种宝交早生的花药愈伤组织建立的悬浮细胞系为试材,对原生质体分离和再生进行研究。结果表明:酶液的渗透压、浓度和配比对悬浮细胞原生质体产量和活力有重要的影响。悬浮细胞在CPW+1.0％ Cellulase R-10+0.5 Macerozyme R-10+0.05％Pectolyase Y-23+0.6mol/L甘露醇+0.5％PVP的酶液中酶解12h,原生质体产量和活力最高,分别达16.35×10~6/g和84.6％。采用液体浅层培养法对原生质体进行培养获得了再生愈伤组织。 |
| 关 键 词： | 草莓 原生质体 培养 愈伤组织 |
| 文章编号： | 1009-9980(2003)04-319-03 |
| 修稿时间： | 2002年12月5日 |
Regeneration of Callus from Protoplasts of Suspension Cells in Strawberry |
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| Zhou Chunjiang,Li Weidong,and Ge Huibo.Regeneration of Callus from Protoplasts of Suspension Cells in Strawberry[J].Journal of Fruit Science,2003,20(4):319-321. | |
| Authors: | Zhou Chunjiang Li Weidong and Ge Huibo |
| Abstract: | The conditions of establishment of suspension cell lines from anther-derived calli, protoplast isolation and regeneration of strawberry Hokowase were studied. Osmotic pressure, concentration and ratio of enzyme solution affected the yield and viability of protoplasts. The protoplast yield and viability could amount to 16.35×106 protoplasts/gFW and 84.6% respectively when suspension cultures were incubated in enzyme solution containing CPW salts, 1.0%Cellulase R-10, 0.5% Macerozyme R-10,0.05%Pectolyase Y-23 ,0.6 mol/L mannitol and 0.5% polyvinylpyrrolidone for 12 hours. Protoplasts were cultured in thin-layer liquid medium, and protoplast-derived calli were obtained. |
| Keywords: | Strawberry(Fragria ananassa Duch ) Protoplast Culture Callus |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |