| 灰霉病菌对三种杀菌剂的抗性表现型分布及稳定性测定 |
| |
| 引用本文： | 韩巨才,刘慧平,闫秀琴,高俊明.灰霉病菌对三种杀菌剂的抗性表现型分布及稳定性测定[J].农药学学报,2004,6(3):43-47. |
| |
| 作者姓名： | 韩巨才 刘慧平 闫秀琴 高俊明 |
| |
| 作者单位： | 1.山西农业大学 农学院, 山西 太谷030801 |
| |
| 基金项目： | 山西省留学基金项目(99051)，山西省科技攻关项目(031011-4，033006). |
| |
| 摘 要： | 采用最低抑制浓度法(MIC)和菌落直径法,对采自山西晋中、晋南、晋东南和晋北4个地区的188个灰霉病菌株对3种杀菌剂的抗性表现型和稳定性进行了测定,旨在了解灰霉病菌的抗药性状况和预测其抗性发展趋势。结果表明:对多菌灵的抗性表现型可分为敏感(S)、低抗或中抗(RM)和高抗(R),对腐霉利和乙霉威可分为敏感(S)和抗性(R)。以对多菌灵、腐霉利和乙霉威的抗性为序,检测出8种不同类型的抗性表现型,即RRR、RRS、RSR、RSS、RMRS、RMSR、RMSS 和SSR。各表现型的分布频率随各地区用药历史和水平的不同而变化,并与抗性监测结果相一致。对55个不同抗性表现型的单孢菌株的稳定性测定结果表明:继代无药培养10代后,以RRR和RSS型表现最稳定,而其余类型菌株均不同程度发生了变异,尤其是RMSS和RMRS型菌株100%发生了变异,抗性表现型不稳定的菌株占52.7%。对多菌灵的抗性变化趋势是由敏感向低抗、再向高抗发展;对腐霉利的抗性变化主要是由抗性转变为敏感;而对乙霉威的抗性则相对比较稳定。
|
| 关 键 词： | 灰霉病菌 杀菌剂 抗性表现型 稳定性 |
| 文章编号： | 1008-7303(2004)03-0043-05 |
| 收稿时间： | 2004/2/26 0:00:00 |
| 修稿时间： | 2004年2月26日 |
Resistance-phenotype and Stability of Botrytis cinerea to Three Fungicides |
| |
| HAN Ju-cai,LIU Hui-ping,YAN Xiu-qin and GAO Jun-ming.Resistance-phenotype and Stability of Botrytis cinerea to Three Fungicides[J].Chinese Journal of Pesticide Science,2004,6(3):43-47. |
| |
| Authors: | HAN Ju-cai LIU Hui-ping YAN Xiu-qin and GAO Jun-ming |
| |
| Institution: | 1.Agronomy College, Shanxi Agricultural University, Taigu 030801, China |
| |
| Abstract: | With MIC and colony diameter methods, the resistance-phenotype and stability of 188 Botrytis cinerea isolates, collected from Jinzhong, Jinnan, Jindongnan and Jinbei regions of Shanxi province, to 3 fungicides were tested for evaluating the resistance situation and predicting the trend of resistancedevelopment. The results showed that resistance-phenotype to carbendazim (Ben) could be divided into sensitive (S), low or moderate (RM) and high resistant (R) groups, whereas to procymidone (Dic) and diethofencarb(NPC) could be classified into sensitive (S) and resistant (R) groups. Eight different resistance-phenotype to three fungicides, MBC, Dic and NPC, have been detected, they were RRR, RRS, RSR, RSS, RMRS, RMSR, RMSS and SSR. The frequency distribution of the resistance-phenotype in different regions is varied with the history and level of fungicide use, and was consistent with the result of resistance monitoring. The results of stability tests of 55 isolates with different resistance-phenotype indicated that after ten generations of subculture in the absence of fungicides, RRR and RSS phenotypes exhibited most stable, but other phenotypes have been mutated to different extent. All of RMSS and RMRS phenotypes mutated, and unstable isolates accounted for 52.7%. The variation trend of resistance to Ben was from sensitive or low-level of resistance to high-level of resistance, whereas the resistance to Dic inclined to vary from resistance to sensitivity, and the resistance to NPC was relatively stable. |
| |
| Keywords: | Botrytis cinerea Pers fungicide resistance-phenotype stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
| 点击此处可从《农药学学报》下载免费的PDF全文 |
|
