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大豆RIL群体ZKS-HX的构建及遗传结构分析
引用本文:吕祝章,张娜,李慷均,常汝镇,邱丽娟.大豆RIL群体ZKS-HX的构建及遗传结构分析[J].河南农业科学,2010(5).
作者姓名:吕祝章  张娜  李慷均  常汝镇  邱丽娟
作者单位:1. 日照职业技术学院,山东,日照,276826
2. 中国农业科学院,作物研究所,国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室,北京,100081
摘    要:选用全国种植时间最长(20多a)且累计种植面积最大(1.3×106hm2)的栽培大豆(G.max)品种合丰25为母本,高蛋白、结荚多的半野生大豆(G.gracilus)新民6号为父本杂交得到F2∶8代122个重组自交系(RIL)群体(ZKS-HX),利用124个SSR标记对大豆RIL群体进行遗传组成分析,结果表明,父、母本对群体的遗传贡献分别为0.529和0.471,2个亲本在群体中的分离比例相近,总体上未出现严重偏离1∶1的分离比例,群体各家系基因型组成近似符合正态分布,适合遗传作图研究。

关 键 词:大豆  重组自交系  遗传结构  SSR

Construction and Genetic Structure Analysis of Soybean Recombinant Inbred Line Population ZKS-HX
L Zhu-zhang,ZHANG Na,LI Kang-jun,CHANG Ru-zhen,QIU Li-juan.Construction and Genetic Structure Analysis of Soybean Recombinant Inbred Line Population ZKS-HX[J].Journal of Henan Agricultural Sciences,2010(5).
Authors:L Zhu-zhang  ZHANG Na  LI Kang-jun  CHANG Ru-zhen  QIU Li-juan
Institution:L(U) Zhu-zhang,ZHANG Na,LI Kang-jun,CHANG Ru-zhen,QIU Li-juan
Abstract:One hundred twenty-two F2∶8 recombinant inbred lines(ZKS-HX) were created by the cross of Hefeng 25 ×Xinmin No.6.Hefeng 25 is the national longest(20 years) planting cultivar and the total cultivated area up to 1.3 million hectares.Xinmin No.6 was a high-protein,semi-wild soybean(G.Gracilus).Genetic composition of the soybean RIL population was analyzed by using 124 SSR markers.The results showed that the genetic contribution of two parents were 0.529 and 0.471 respectively,the two parental separation in groups similar to the proportion of the whole,no serious deviation from 1∶1 segregation ratio was observed.The community various pedigrees genotype composition approximate match normal distribution,suits the heredity mapping research.
Keywords:SSR
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