| 二倍体马铃薯高效再生体系的建立 |
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| 引用本文： | 晁祥健,杨煜,金黎平,张煜,刘生祥,丁汉凤,屈冬玉,李广存.二倍体马铃薯高效再生体系的建立[J].园艺学报,2009,36(1):109-114. |
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| 作者姓名： | 晁祥健 杨煜 金黎平 张煜 刘生祥 丁汉凤 屈冬玉 李广存 |
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| 作者单位： | （山东省农业科学院高新技术研究中心，济南 250100；宁夏大学农学院，银川 750021；中国农业科学院蔬菜花卉研究所，北京 100081） |
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| 基金项目： | 国家高技术研究发展计划(863计划)，山东省自然科学基金，山东省良种工程项目，山东省农业科学院青年基金 |
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| 摘 要： | 以二倍体马铃薯抗青枯病基因型ED13、CE171和感青枯病基因型ED25的茎段为外植体，在优化植物生长调节剂配比的基础上，对不同基因型的组培再生进行了研究。结果表明：不同基因型具有不同生长调节剂配比要求。固体－液体培养基双层看护培养对于二倍体马铃薯茎段愈伤组织的诱导是有效的，尤以固体培养基中NAA 2.0 mg · L-1 : 6-BA 2.0 mg · L-1的配比为佳。对于ED13而言，液体培养基中以2,4-D 2.5 mg · L-1 : KT 0.5 mg · L-1的配比为佳，相比之下CE171则以2,4-D 1.5 mg · L-1 : KT 1.0 mg · L-1为最好，二者的愈伤诱导率均达100%；对于基因型ED25，只有在NAA 2.0 mg · L-1 : 6-BA 1.0 mg · L-1和2,4-D 1.5 mg · L-1 : KT 1.0 mg · L-1时才能形成较好的愈伤。ZT在愈伤组织诱导芽分化过程中具有重要作用，单独使用ZT浓度为1.0 mg · L-1时，ED13和CE171的芽分化率均达95％以上；而ED25则只有在ZT和6-BA配合使用，配比为ZT 0.5 mg · L-1 : 6-BA 2.5 mg · L-1时才可获得较高的芽分化率。
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| 关 键 词： | 二倍体 马铃薯 茎段 愈伤组织 分化 植株再生 |
| 收稿时间： | 2008-8-1 |
| 修稿时间： | 2008-11-4 |
Construction of High Regeneration Systems for Diploid Potato |
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| CHAO Xiang-jian,YANG Yu,JIN Li-ping,ZHANG Yu,LIU Sheng-xiang,DING Han-feng,QU Dong-yu,LI Guang-cun.Construction of High Regeneration Systems for Diploid Potato[J].Acta Horticulturae Sinica,2009,36(1):109-114. |
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| Authors: | CHAO Xiang-jian YANG Yu JIN Li-ping ZHANG Yu LIU Sheng-xiang DING Han-feng QU Dong-yu LI Guang-cun |
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| Institution: | (High-tech Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China; Agriculture College of Ningxia University, Yinchuan 750021, China; nstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081) |
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| Abstract: | High regeneration system for diploid potato was developed using segments as explants of in vitro plantlets of three genotypes, ED13 (bacterial wilt resistant), CE171 (bacterial wilt resistant), and ED25 (bacterial wilt susceptible). The role of plant hormones and their combinations was studied for callus formation and shoot differentiation. The result showed that different genotype needed different hormone combination for its callus formation and shoot differentiation. Solid-liquid nurse medium was helpful for callus induction of diploid potato and the best hormone combination in solid medium was NAA (2.0 mg · L-1): 6-BA (2.0 mg · L-1). For ED13, the best hormone combination in liquid medium was 2,4-D (2.5 mg · L-1) : KT (0.5 mg · L-1); whereas, for CE171, combination of 2,4-D (1.5 mg · L-1) : KT (1.0 mg · L-1) was the best. Both of these medium combinations gave high callus induction up to100%. But for genotype ED25, only the combinations of NAA (2.0 mg · L-1) : 6-BA (1.0 mg · L-1) and 2,4-D (1.5 mg · L-1) : KT (1.0 mg · L-1) could give good callus induction result. ZT played an important role in the induction of shoot differentiation. More than 95% explants of genotype ED13 and CE171 were differentiated using only ZT (1.0 mg · L-1). However, the combination of ZT (0.5 mg · L-1): 6-BA (2.5 mg · L-1) was needed for high shoot differentiation of ED25. |
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| Keywords: | diploid potato segment callus differentiation plant regeneration |
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