| 16个早实核桃良种遗传多样性的FISH-AFLP分析 |
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| 引用本文： | 马庆国,齐静,裴东.16个早实核桃良种遗传多样性的FISH-AFLP分析[J].林业科学研究,2010,23(5):631-636. |
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| 作者姓名： | 马庆国 齐静 裴东 |
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| 作者单位： | 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 |
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| 基金项目： | "十一五"国家科技支撑计划项目(2006BAD01A1703) |
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| 摘 要： | 采用FISH-AFLP技术,筛选9对E+3和M+3引物组合,对我国首批16个早实核桃良种进行了基因组DNA水平上的检测,结果表明:共获得1 072条可统计的条带,其中946条呈多态性,平均的多态性带百分率达88.12%;经9对引物检测的16个品种基因型各不相同,均得到数目不等的特征带,并能将16个早实核桃良种完全区分开。研究分析了早实核桃良种的遗传多样性和亲缘关系并建立了它们的指纹图谱。
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| 关 键 词： | 核桃 遗传多样性 亲缘关系 FISH-AFLP 指纹图谱 |
| 收稿时间： | 2008/11/18 0:00:00 |
FISH-AFLP Analysis of Genetic Diversity of Early-fruiting Walnut Cultivars |
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| MA Qing-guo,QI Jing and PEI Dong.FISH-AFLP Analysis of Genetic Diversity of Early-fruiting Walnut Cultivars[J].Forest Research,2010,23(5):631-636. |
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| Authors: | MA Qing-guo QI Jing and PEI Dong |
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| Institution: | Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
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| Abstract: | 16 early-fruiting walnut (Juglans regia L.) cultivars were developed in China in 1994. These new walnut cultivars preliminarily formed an improved variety system for Chinese walnut production. The FISH-AFLP analysis was conducted in order to investigate the sibship among the 16 Chinese early-fruiting walnut cultivars. 9 primer combinations were used to amplify the genomic DNAs. Total 1 072 AFLP fragments were obtained, in which 946 are polymorphic markers and the average percent of polymorphic bands is 88.12%. Different genotypes were detected and unique bands were found. The genetic diversity and their sibship among Chinese early-fruiting walnut cultivars were investigated and their fingerprintings were established in the research. |
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| Keywords: | Walnut Juglans regia genetic diversity sibship FISH-AFLP fingerprinting |
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