| 苏云金杆菌菌种保藏 |
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| 引用本文： | 陈在佴,张汉珍,吴继星.苏云金杆菌菌种保藏[J].中国生物防治,1999,15(1):19-22. |
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| 作者姓名： | 陈在佴 张汉珍 吴继星 |
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| 作者单位： | 湖北省农科院Bt研究开发中心,武汉,430064 |
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| 基金项目： | 国家“七五”、“八五”攻关项目 |
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| 摘 要： | 用８种方法保藏１８个Ｂｔ菌株５～２３年，结果表明，不同保藏方法对Ｂｔ菌株的存活率和毒效存在不同程度的影响。这些菌株平均存活率为２９．３％～８０．３％。除保藏５年的ＭＰ┐３４２外，较高者有７９００７（１８年）５７．５％，８２６１２（１５年）５６．９％；最低为０１６（２２年）２９．３％。不同保藏方法平均存活率排序：土壤保藏Ａ（５５．５％）＞沙管保藏Ａ（５３．０％）＞虫尸保藏Ａ（４７．５％）＞土壤保藏Ｂ（４３．５％）＞沙管保藏Ｂ（３８．７％）＞虫尸保藏Ｂ（３６．６％）＞石蜡保藏（３１．６％）＞滤纸保藏（３０．１％）。沙管、土壤和虫尸３种方法适用于Ｂｔ的菌种保藏
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| 关 键 词： | 苏云金杆菌 存活率 毒效 菌种保藏 |
| 修稿时间： | 1998-04-21 |
Culture Conservation of Bacillus thuringiensis |
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| CHEN Zaier,ZHANG Hanzhen,WU Jixing.Culture Conservation of Bacillus thuringiensis[J].Chinese Journal of Biological Control,1999,15(1):19-22. |
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| Authors: | CHEN Zaier ZHANG Hanzhen WU Jixing |
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| Abstract: | methods for preservation of Bt cultures were tested through a period of 5-23 years The 18 Bt strains showed different survival rates, averaging 29 3%-80 3% after storage The survival rates for strain 79007 was 57 5% (18 years), for strain 82612 was 56 9% (15 years), for strain 016 was 29 3% (22 years) The average survival rates for different storage methods were soil tube A method (55 5%), sand tube A method (53 0%), insect corpse A method (47 5%), soil tube B method (43 5%), sand tube B method (38 7%), insect corpse B method (36 6%), paraffin wax tube method (31 6%) and filter paper tube method (30 1%) The sand tube, soil tube and insect corpse tube methods could be used for the preservation of Bt culture |
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