| 广西地区水牛乳微量元素含量及其影响因素研究 |
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| 引用本文： | 喻笑男,刘宏艳,郭晓青,赵青余,李发弟,张军民.广西地区水牛乳微量元素含量及其影响因素研究[J].中国畜牧兽医,2020,47(1):296-305. |
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| 作者姓名： | 喻笑男 刘宏艳 郭晓青 赵青余 李发弟 张军民 |
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| 作者单位： | 1. 兰州大学草地农业科技学院, 兰州 730000;2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 农业农村部华北动物遗传资源与营养科学观测实验站, 北京 100193 |
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| 基金项目： | 国家科技支撑计划项目(2014BAD13B05-03);中国农业科学院科技创新工程协同创新任务(CAAS-XTCX2016011-01);中国农业科学院科技创新工程(ASTIP-IAS12) |
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| 摘 要： | 水牛乳是中国南方地区重要奶源，为了解中国广西地区水牛乳中微量元素含量及不同因素如地域、品种和泌乳期对水牛乳中微量元素含量的影响，于广西南宁市兴宁区、邕宁区和钦州市灵山县共3个水牛场采集了摩拉、尼里2个品种，3个泌乳时期的水牛乳样品共60份，通过微波消解，利用电感耦合等离子体质谱检测了铬（Cr）、锰（Mn）、钴（Co）、铜（Cu）、砷（As）、硒（Se）、铯（Cs）、钡（Ba）、钼（Mo）、铅（Pb）、镉（Cd）、镍（Ni）共12种微量元素的含量，采用皮尔逊相关分析解析了12种元素含量之间的相关性，采用单因素方差分析比较了不同地域、品种和泌乳期水牛乳之间微量元素含量差异，采用多因素方差分析解析交互作用对元素含量的影响。结果表明，该法测定水牛乳12种微量元素含量的加标回收率在84.94%~120.91%之间。不同地域间水牛乳中Mn、Se、Cs、Ni含量差异显著（P<0.05），钦州市灵山县水牛乳中Mn、Cs含量较高，南宁市兴宁区水牛乳中Se、Ni含量较高；不同品种间水牛乳中Se、Ni含量差异显著（P<0.05），尼里水牛乳中Se、Ni含量高于摩拉水牛；不同泌乳期水牛乳中Mn、Co、Cu、Se、Mo含量差异显著（P<0.05），初乳中Co、Cu、Se含量较高，常乳中Mn含量较高，晚乳中Mo含量较高；地域与品种的交互作用对水牛乳中微量元素含量无显著影响。
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| 关 键 词： | 水牛乳 微量元素 地域 品种 泌乳期 |
| 收稿时间： | 2019-07-22 |
Study on the Contents of Trace Elements and the Influencing Factors in Buffalo Milk in Guangxi |
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| YU Xiaonan,LIU Hongyan,GUO xiaoqing,ZHAO Qingyu,LI Fadi,ZHANG Junmin.Study on the Contents of Trace Elements and the Influencing Factors in Buffalo Milk in Guangxi[J].China Animal Husbandry & Veterinary Medicine,2020,47(1):296-305. |
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| Authors: | YU Xiaonan LIU Hongyan GUO xiaoqing ZHAO Qingyu LI Fadi ZHANG Junmin |
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| Institution: | 1. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China;2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture and Rural Affairs, Beijing 100193, China |
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| Abstract: | Buffalo milk was an important milk source in the South of China.In order to study the contents of trace elements in buffalo milk in Guangxi and the influence of different factors such as region,breed,lactation,60 samples of buffalo milk with 2 breeds in 3 lactation periods were collected from 3 buffalo farms in Xingning and Yongning district in Nanning and Lingshan district in Qinzhou,Guangxi province.The samples were digested by microwave digestion method.12 trace elements (Cr,Mn,Co,Cu,As,Se,Cs,Ba,Mo,Pb,Cd and Ni) were analyzed by inductively coupled plasma mass spectrometry method (ICP-MS).Correlation among 12 trace element contents was analyzed using Pearson correlation analysis.Differences in trace element contents between buffalo milk in different regions,breeds and lactation stages were compared by one-way analysis of variance.Multivariate analysis of variance was used to analyze the effect of interaction on trace elemental contents.The results showed that the spiked recovery of 12 trace elements in buffalo milk was 84.94% to 120.91%.The contents of Mn,Se,Cs and Ni in buffalo milk were significantly different among different regions (P<0.05).The contents of Mn and Cs in Lingshan were higher than those in other regions.The contents of Se and Ni in Xingning were higher than those in other region.The contents of Se and Ni in buffalo milk were significantly different between different breeds (P<0.05).The contents of Se and Ni in Nile buffalo were higher than that in Mora buffalo.The contents of Mn,Co,Cu,Se and Mo were significantly different among different lactation periods (P<0.05).The contents of Co,Cu and Se in colostrum were the highest among three lactation periods.The content of Mn in mature milk was higher than that in other lactation periods.The content of Mo in late milk was the highest among three lactation periods.The interaction between region and breed had no significant effect on the contents of trace elements in buffalo milk. |
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| Keywords: | buffalo milk trace element region variety lactation |
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